Immunocytochemical detection of the 70-kd heat shock protein in alcoholic liver disease

R. Omar, Miguel Pappolla, B. Saran

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Exposure of cells to physical (eg, heat) or chemical (eg, alcohol) stress results in increased synthesis of a set of highly conserved polypeptides termed heat shock proteins (HSPs), among which the 70-kd protein (HSP 70) is one of the most consistently inducible and highly conserved. This HSP has adenosine triphosphate-binding properties and is known to associate strongly with cytoskeletal structures that are usually disrupted on injury by heat or alcohol. Some HSPs apparently function as accessories to a nonlysosomal, adenosine triphosphate-dependent proteolytic system that binds and digests away stress-generated abnormal or denatured proteins after their conjugation with ubiquitin, a small HSP. Ubiquitin has been demonstrated immunocytochemically in Mallory bodies, which represent mainly degenerated intermediate filaments accumulated in hepatocytes of alcoholic-diseased liver. We immunostained histologic sections from patients with alcoholic liver disease using a polyclonal antibody raised against HSP 70. Strong diffuse cytoplasmic immunoreactivity was observed in many hepatocytes, including cells without Mallory bodies or fatty degeneration. Positive immunoreactivity for HSP 70 points to a possible involvement of this HSP in the pathogenesis of alcoholic liver disease. It also suggests that immunocytochemical detection of HSP 70 may serve as a more sensitive indicator of hepatocellular injury.

Original languageEnglish (US)
Pages (from-to)589-592
Number of pages4
JournalArchives of Pathology and Laboratory Medicine
Volume114
Issue number6
StatePublished - 1990
Externally publishedYes

Fingerprint

Alcoholic Liver Diseases
HSP70 Heat-Shock Proteins
Heat-Shock Proteins
Mallory Bodies
Ubiquitin
Hepatocytes
ATP-Dependent Proteases
Hot Temperature
Alcohols
Small Heat-Shock Proteins
Intermediate Filaments
Wounds and Injuries
Proteins
Adenosine Triphosphate
Peptides
Antibodies

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Medical Laboratory Technology

Cite this

Immunocytochemical detection of the 70-kd heat shock protein in alcoholic liver disease. / Omar, R.; Pappolla, Miguel; Saran, B.

In: Archives of Pathology and Laboratory Medicine, Vol. 114, No. 6, 1990, p. 589-592.

Research output: Contribution to journalArticle

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