Immunocytochemical localization of laminin in hamster tracheal epithelial cell cultures

P. C. Moller, J. S. Bergmann, M. J. Evans, B. W. Weaver, R. L. Given, T. N. Blankenship

    Research output: Contribution to journalArticle

    4 Citations (Scopus)

    Abstract

    EM examination of 28 day cultures of enzymatically dissociated hamster tracheal epithelial (HTE) cells grown on collagen coated millipore filters reveals that fragments of basal lamina may be present at the basal plasmalemma. Since the basal lamina consists of several major components including type IV collagen, heparan sulfate proteoglycans, entactin/nidogen, and laminin, questions naturally arise concerning the presence of such a structure in this cell culture system. When immunocytochemical procedures utilizing anti-laminin antibody and PAP techniques are carried out with paraffin sections of HTE culture at 1, 2,3, and 4 weeks in vitro, LM analysis reveals that a thin, dense line of reaction product is present between the basal surface of the HTE cells and the underlying collagen substrate. Immunoblotting evaluation carried out with supernatants of 7d HTE cell homogenates and HTE cell conditioned media also indicate that laminin is being produced by the tracheal cells. Thus, the presence of basal lamina-like fragments, the immunocytochemical localization of laminin, and immunoblot identification of laminin in hamster tracheal epithelial cell cultures, suggest that, although basal lamina components may be produced by HTE cells, at the time points tested, they are not yet being organized into a complete basal lamina.

    Original languageEnglish (US)
    Pages (from-to)427-435
    Number of pages9
    JournalTissue and Cell
    Volume23
    Issue number4
    DOIs
    StatePublished - 1991

    Fingerprint

    laminin
    Laminin
    hamsters
    Cricetinae
    cell culture
    epithelial cells
    Cell Culture Techniques
    Epithelial Cells
    Basement Membrane
    collagen
    Collagen
    Micropore Filters
    Heparan Sulfate Proteoglycans
    Collagen Type IV
    proteoglycans
    Conditioned Culture Medium
    immunoblotting
    Immunoblotting
    Paraffin
    alkanes

    Keywords

    • epithelia
    • immunocytochemistry
    • laminin
    • Tissue culture
    • trachea

    ASJC Scopus subject areas

    • Insect Science
    • Cell Biology

    Cite this

    Moller, P. C., Bergmann, J. S., Evans, M. J., Weaver, B. W., Given, R. L., & Blankenship, T. N. (1991). Immunocytochemical localization of laminin in hamster tracheal epithelial cell cultures. Tissue and Cell, 23(4), 427-435. https://doi.org/10.1016/0040-8166(91)90001-A

    Immunocytochemical localization of laminin in hamster tracheal epithelial cell cultures. / Moller, P. C.; Bergmann, J. S.; Evans, M. J.; Weaver, B. W.; Given, R. L.; Blankenship, T. N.

    In: Tissue and Cell, Vol. 23, No. 4, 1991, p. 427-435.

    Research output: Contribution to journalArticle

    Moller, PC, Bergmann, JS, Evans, MJ, Weaver, BW, Given, RL & Blankenship, TN 1991, 'Immunocytochemical localization of laminin in hamster tracheal epithelial cell cultures', Tissue and Cell, vol. 23, no. 4, pp. 427-435. https://doi.org/10.1016/0040-8166(91)90001-A
    Moller, P. C. ; Bergmann, J. S. ; Evans, M. J. ; Weaver, B. W. ; Given, R. L. ; Blankenship, T. N. / Immunocytochemical localization of laminin in hamster tracheal epithelial cell cultures. In: Tissue and Cell. 1991 ; Vol. 23, No. 4. pp. 427-435.
    @article{b688ed056b4547959cea0551d50c6069,
    title = "Immunocytochemical localization of laminin in hamster tracheal epithelial cell cultures",
    abstract = "EM examination of 28 day cultures of enzymatically dissociated hamster tracheal epithelial (HTE) cells grown on collagen coated millipore filters reveals that fragments of basal lamina may be present at the basal plasmalemma. Since the basal lamina consists of several major components including type IV collagen, heparan sulfate proteoglycans, entactin/nidogen, and laminin, questions naturally arise concerning the presence of such a structure in this cell culture system. When immunocytochemical procedures utilizing anti-laminin antibody and PAP techniques are carried out with paraffin sections of HTE culture at 1, 2,3, and 4 weeks in vitro, LM analysis reveals that a thin, dense line of reaction product is present between the basal surface of the HTE cells and the underlying collagen substrate. Immunoblotting evaluation carried out with supernatants of 7d HTE cell homogenates and HTE cell conditioned media also indicate that laminin is being produced by the tracheal cells. Thus, the presence of basal lamina-like fragments, the immunocytochemical localization of laminin, and immunoblot identification of laminin in hamster tracheal epithelial cell cultures, suggest that, although basal lamina components may be produced by HTE cells, at the time points tested, they are not yet being organized into a complete basal lamina.",
    keywords = "epithelia, immunocytochemistry, laminin, Tissue culture, trachea",
    author = "Moller, {P. C.} and Bergmann, {J. S.} and Evans, {M. J.} and Weaver, {B. W.} and Given, {R. L.} and Blankenship, {T. N.}",
    year = "1991",
    doi = "10.1016/0040-8166(91)90001-A",
    language = "English (US)",
    volume = "23",
    pages = "427--435",
    journal = "Tissue and Cell",
    issn = "0040-8166",
    publisher = "Churchill Livingstone",
    number = "4",

    }

    TY - JOUR

    T1 - Immunocytochemical localization of laminin in hamster tracheal epithelial cell cultures

    AU - Moller, P. C.

    AU - Bergmann, J. S.

    AU - Evans, M. J.

    AU - Weaver, B. W.

    AU - Given, R. L.

    AU - Blankenship, T. N.

    PY - 1991

    Y1 - 1991

    N2 - EM examination of 28 day cultures of enzymatically dissociated hamster tracheal epithelial (HTE) cells grown on collagen coated millipore filters reveals that fragments of basal lamina may be present at the basal plasmalemma. Since the basal lamina consists of several major components including type IV collagen, heparan sulfate proteoglycans, entactin/nidogen, and laminin, questions naturally arise concerning the presence of such a structure in this cell culture system. When immunocytochemical procedures utilizing anti-laminin antibody and PAP techniques are carried out with paraffin sections of HTE culture at 1, 2,3, and 4 weeks in vitro, LM analysis reveals that a thin, dense line of reaction product is present between the basal surface of the HTE cells and the underlying collagen substrate. Immunoblotting evaluation carried out with supernatants of 7d HTE cell homogenates and HTE cell conditioned media also indicate that laminin is being produced by the tracheal cells. Thus, the presence of basal lamina-like fragments, the immunocytochemical localization of laminin, and immunoblot identification of laminin in hamster tracheal epithelial cell cultures, suggest that, although basal lamina components may be produced by HTE cells, at the time points tested, they are not yet being organized into a complete basal lamina.

    AB - EM examination of 28 day cultures of enzymatically dissociated hamster tracheal epithelial (HTE) cells grown on collagen coated millipore filters reveals that fragments of basal lamina may be present at the basal plasmalemma. Since the basal lamina consists of several major components including type IV collagen, heparan sulfate proteoglycans, entactin/nidogen, and laminin, questions naturally arise concerning the presence of such a structure in this cell culture system. When immunocytochemical procedures utilizing anti-laminin antibody and PAP techniques are carried out with paraffin sections of HTE culture at 1, 2,3, and 4 weeks in vitro, LM analysis reveals that a thin, dense line of reaction product is present between the basal surface of the HTE cells and the underlying collagen substrate. Immunoblotting evaluation carried out with supernatants of 7d HTE cell homogenates and HTE cell conditioned media also indicate that laminin is being produced by the tracheal cells. Thus, the presence of basal lamina-like fragments, the immunocytochemical localization of laminin, and immunoblot identification of laminin in hamster tracheal epithelial cell cultures, suggest that, although basal lamina components may be produced by HTE cells, at the time points tested, they are not yet being organized into a complete basal lamina.

    KW - epithelia

    KW - immunocytochemistry

    KW - laminin

    KW - Tissue culture

    KW - trachea

    UR - http://www.scopus.com/inward/record.url?scp=0025912817&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=0025912817&partnerID=8YFLogxK

    U2 - 10.1016/0040-8166(91)90001-A

    DO - 10.1016/0040-8166(91)90001-A

    M3 - Article

    C2 - 1926135

    AN - SCOPUS:0025912817

    VL - 23

    SP - 427

    EP - 435

    JO - Tissue and Cell

    JF - Tissue and Cell

    SN - 0040-8166

    IS - 4

    ER -