Immunodiagnosis of Ehrlichia canis infection with recombinant proteins

Jere McBride, R. E. Corstvet, E. B. Breitschwerdt, David Walker

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Ehrlichia canis causes a potentially fatal rickettsial disease of dogs that requires rapid and accurate diagnosis in order to initiate appropriate therapy leading to a favorable prognosis. We recently reported the cloning of two immunoreactive E. canis proteins, P28 and P140, that were applicable for serodiagnosis of the disease. In the present study we cloned a new immunoreactive E. canis surface protein gene of 1,170 bp, which encodes a protein with a predicted molecular mass of 42.6 kDa (P43). The P43 gene was not detected in E. chaffeensis DNA by Southern blot, and antisera against recombinant P43 (rP43) did not react with E. chaffeensis as detected by indirect fluorescent antibody (IFA) assay. Forty-two dogs exhibiting signs and/or hematologic abnormalities associated with canine ehrlichiosis were tested by IFA assay and by recombinant Western immunoblot. Among the 22 samples that were IFA positive for E. canis, 100% reacted with rP43, 96% reacted with rP28, and 96% reacted with rP140. The specificity of the recombinant proteins compared to the IFAs was 96% for rP28, 88% for P43 and 63% for P140. The results of this study demonstrate that the rP43 and rP28 are sensitive and reliable serodiagnostic antigens for E. canis infections.

Original languageEnglish (US)
Pages (from-to)315-322
Number of pages8
JournalJournal of Clinical Microbiology
Volume39
Issue number1
DOIs
StatePublished - 2001

Fingerprint

Ehrlichia canis
Immunologic Tests
Recombinant Proteins
Infection
Antibodies
Ehrlichiosis
Dog Diseases
Serologic Tests
Southern Blotting
Genes
Canidae
Organism Cloning
Immune Sera
Membrane Proteins
Proteins
Western Blotting
Dogs
Antigens
DNA

ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology

Cite this

Immunodiagnosis of Ehrlichia canis infection with recombinant proteins. / McBride, Jere; Corstvet, R. E.; Breitschwerdt, E. B.; Walker, David.

In: Journal of Clinical Microbiology, Vol. 39, No. 1, 2001, p. 315-322.

Research output: Contribution to journalArticle

McBride, Jere ; Corstvet, R. E. ; Breitschwerdt, E. B. ; Walker, David. / Immunodiagnosis of Ehrlichia canis infection with recombinant proteins. In: Journal of Clinical Microbiology. 2001 ; Vol. 39, No. 1. pp. 315-322.
@article{27283643699f41bfbb0a69fc72f0e566,
title = "Immunodiagnosis of Ehrlichia canis infection with recombinant proteins",
abstract = "Ehrlichia canis causes a potentially fatal rickettsial disease of dogs that requires rapid and accurate diagnosis in order to initiate appropriate therapy leading to a favorable prognosis. We recently reported the cloning of two immunoreactive E. canis proteins, P28 and P140, that were applicable for serodiagnosis of the disease. In the present study we cloned a new immunoreactive E. canis surface protein gene of 1,170 bp, which encodes a protein with a predicted molecular mass of 42.6 kDa (P43). The P43 gene was not detected in E. chaffeensis DNA by Southern blot, and antisera against recombinant P43 (rP43) did not react with E. chaffeensis as detected by indirect fluorescent antibody (IFA) assay. Forty-two dogs exhibiting signs and/or hematologic abnormalities associated with canine ehrlichiosis were tested by IFA assay and by recombinant Western immunoblot. Among the 22 samples that were IFA positive for E. canis, 100{\%} reacted with rP43, 96{\%} reacted with rP28, and 96{\%} reacted with rP140. The specificity of the recombinant proteins compared to the IFAs was 96{\%} for rP28, 88{\%} for P43 and 63{\%} for P140. The results of this study demonstrate that the rP43 and rP28 are sensitive and reliable serodiagnostic antigens for E. canis infections.",
author = "Jere McBride and Corstvet, {R. E.} and Breitschwerdt, {E. B.} and David Walker",
year = "2001",
doi = "10.1128/JCM.39.1.315-322.2001",
language = "English (US)",
volume = "39",
pages = "315--322",
journal = "Journal of Clinical Microbiology",
issn = "0095-1137",
publisher = "American Society for Microbiology",
number = "1",

}

TY - JOUR

T1 - Immunodiagnosis of Ehrlichia canis infection with recombinant proteins

AU - McBride, Jere

AU - Corstvet, R. E.

AU - Breitschwerdt, E. B.

AU - Walker, David

PY - 2001

Y1 - 2001

N2 - Ehrlichia canis causes a potentially fatal rickettsial disease of dogs that requires rapid and accurate diagnosis in order to initiate appropriate therapy leading to a favorable prognosis. We recently reported the cloning of two immunoreactive E. canis proteins, P28 and P140, that were applicable for serodiagnosis of the disease. In the present study we cloned a new immunoreactive E. canis surface protein gene of 1,170 bp, which encodes a protein with a predicted molecular mass of 42.6 kDa (P43). The P43 gene was not detected in E. chaffeensis DNA by Southern blot, and antisera against recombinant P43 (rP43) did not react with E. chaffeensis as detected by indirect fluorescent antibody (IFA) assay. Forty-two dogs exhibiting signs and/or hematologic abnormalities associated with canine ehrlichiosis were tested by IFA assay and by recombinant Western immunoblot. Among the 22 samples that were IFA positive for E. canis, 100% reacted with rP43, 96% reacted with rP28, and 96% reacted with rP140. The specificity of the recombinant proteins compared to the IFAs was 96% for rP28, 88% for P43 and 63% for P140. The results of this study demonstrate that the rP43 and rP28 are sensitive and reliable serodiagnostic antigens for E. canis infections.

AB - Ehrlichia canis causes a potentially fatal rickettsial disease of dogs that requires rapid and accurate diagnosis in order to initiate appropriate therapy leading to a favorable prognosis. We recently reported the cloning of two immunoreactive E. canis proteins, P28 and P140, that were applicable for serodiagnosis of the disease. In the present study we cloned a new immunoreactive E. canis surface protein gene of 1,170 bp, which encodes a protein with a predicted molecular mass of 42.6 kDa (P43). The P43 gene was not detected in E. chaffeensis DNA by Southern blot, and antisera against recombinant P43 (rP43) did not react with E. chaffeensis as detected by indirect fluorescent antibody (IFA) assay. Forty-two dogs exhibiting signs and/or hematologic abnormalities associated with canine ehrlichiosis were tested by IFA assay and by recombinant Western immunoblot. Among the 22 samples that were IFA positive for E. canis, 100% reacted with rP43, 96% reacted with rP28, and 96% reacted with rP140. The specificity of the recombinant proteins compared to the IFAs was 96% for rP28, 88% for P43 and 63% for P140. The results of this study demonstrate that the rP43 and rP28 are sensitive and reliable serodiagnostic antigens for E. canis infections.

UR - http://www.scopus.com/inward/record.url?scp=0035161271&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035161271&partnerID=8YFLogxK

U2 - 10.1128/JCM.39.1.315-322.2001

DO - 10.1128/JCM.39.1.315-322.2001

M3 - Article

VL - 39

SP - 315

EP - 322

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

SN - 0095-1137

IS - 1

ER -