Immunofluorescent analysis of expression of the RNA tumor virus major glycoprotein, gp71, on surfaces of virus producing murine and other mammalian species cell lines

M. W. Cloyd, D. P. Bolognesi, D. D. Bigner

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The specificity of a single rabbit antiserum pool raised against the purified major glycoprotein, gp71, of Friend murine leukemia virus was determined for a variety of virus producing mouse, feline, and gibbon ape cell lines by viable cell membrane immunofluorescence absorption. Among murine cells examined, Friend gp71 type specificity was shared only with Rauscher virus producing cells, and a group specificity was present for all the murine leukemia virus producing cells tested. Friend and Rauscher murine leukemia virus infected cells shared interspecies crossreactivity with feline leukemia and gibbon ape lymphoma virus producing cells. However, Moloney, Gross, and other virus producing murine cells shared some, but not all, of these gp71 interspecies determinants with the feline and primate cells. Immunoferritin electron microscopy localized these gp71 antigenic determinants on both virus and cells membranes.

Original languageEnglish (US)
Pages (from-to)922-930
Number of pages9
JournalCancer Research
Volume37
Issue number3
StatePublished - 1977

Fingerprint

Retroviridae
Glycoproteins
Viruses
Cell Line
Rauscher Virus
Hylobates
Friend murine leukemia virus
Felidae
Hominidae
Feline Leukemia
AKR murine leukemia virus
Cell Membrane
Murine Leukemia Viruses
Primates
Fluorescent Antibody Technique
Immune Sera
Epitopes
Lymphoma
Electron Microscopy
Rabbits

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Immunofluorescent analysis of expression of the RNA tumor virus major glycoprotein, gp71, on surfaces of virus producing murine and other mammalian species cell lines. / Cloyd, M. W.; Bolognesi, D. P.; Bigner, D. D.

In: Cancer Research, Vol. 37, No. 3, 1977, p. 922-930.

Research output: Contribution to journalArticle

@article{1db963494adc48c3a699953d0d1ccecb,
title = "Immunofluorescent analysis of expression of the RNA tumor virus major glycoprotein, gp71, on surfaces of virus producing murine and other mammalian species cell lines",
abstract = "The specificity of a single rabbit antiserum pool raised against the purified major glycoprotein, gp71, of Friend murine leukemia virus was determined for a variety of virus producing mouse, feline, and gibbon ape cell lines by viable cell membrane immunofluorescence absorption. Among murine cells examined, Friend gp71 type specificity was shared only with Rauscher virus producing cells, and a group specificity was present for all the murine leukemia virus producing cells tested. Friend and Rauscher murine leukemia virus infected cells shared interspecies crossreactivity with feline leukemia and gibbon ape lymphoma virus producing cells. However, Moloney, Gross, and other virus producing murine cells shared some, but not all, of these gp71 interspecies determinants with the feline and primate cells. Immunoferritin electron microscopy localized these gp71 antigenic determinants on both virus and cells membranes.",
author = "Cloyd, {M. W.} and Bolognesi, {D. P.} and Bigner, {D. D.}",
year = "1977",
language = "English (US)",
volume = "37",
pages = "922--930",
journal = "Journal of Cancer Research",
issn = "0008-5472",
publisher = "American Association for Cancer Research Inc.",
number = "3",

}

TY - JOUR

T1 - Immunofluorescent analysis of expression of the RNA tumor virus major glycoprotein, gp71, on surfaces of virus producing murine and other mammalian species cell lines

AU - Cloyd, M. W.

AU - Bolognesi, D. P.

AU - Bigner, D. D.

PY - 1977

Y1 - 1977

N2 - The specificity of a single rabbit antiserum pool raised against the purified major glycoprotein, gp71, of Friend murine leukemia virus was determined for a variety of virus producing mouse, feline, and gibbon ape cell lines by viable cell membrane immunofluorescence absorption. Among murine cells examined, Friend gp71 type specificity was shared only with Rauscher virus producing cells, and a group specificity was present for all the murine leukemia virus producing cells tested. Friend and Rauscher murine leukemia virus infected cells shared interspecies crossreactivity with feline leukemia and gibbon ape lymphoma virus producing cells. However, Moloney, Gross, and other virus producing murine cells shared some, but not all, of these gp71 interspecies determinants with the feline and primate cells. Immunoferritin electron microscopy localized these gp71 antigenic determinants on both virus and cells membranes.

AB - The specificity of a single rabbit antiserum pool raised against the purified major glycoprotein, gp71, of Friend murine leukemia virus was determined for a variety of virus producing mouse, feline, and gibbon ape cell lines by viable cell membrane immunofluorescence absorption. Among murine cells examined, Friend gp71 type specificity was shared only with Rauscher virus producing cells, and a group specificity was present for all the murine leukemia virus producing cells tested. Friend and Rauscher murine leukemia virus infected cells shared interspecies crossreactivity with feline leukemia and gibbon ape lymphoma virus producing cells. However, Moloney, Gross, and other virus producing murine cells shared some, but not all, of these gp71 interspecies determinants with the feline and primate cells. Immunoferritin electron microscopy localized these gp71 antigenic determinants on both virus and cells membranes.

UR - http://www.scopus.com/inward/record.url?scp=0017689227&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0017689227&partnerID=8YFLogxK

M3 - Article

C2 - 65219

AN - SCOPUS:0017689227

VL - 37

SP - 922

EP - 930

JO - Journal of Cancer Research

JF - Journal of Cancer Research

SN - 0008-5472

IS - 3

ER -