Immunofluorescent analysis of expression of the RNAT tumor virus major glycoprotein, gp71, on the surfaces of normal murine cells

M. W. Cloyd, D. P. Bolognesi, D. D. Bigner

Research output: Contribution to journalArticle

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Abstract

The expression of the major glycoprotein, gp71, of murine leukemia virus was studied on the surfaces of a variety of normal murine cell lines with a monospecific rabbit antiserum raised against purified Friend murine leukemia virus gp71. Using viable cell membrane immunofluorescence, most established and early passage normal murine cell lines were significantly reactive with the antiserum, irrespective of neoplastic transformation, strain genotype, or whether they were of embryonic or adult tissue origin. The only murine cells tested not detectably expressing gp71 determinants were BALB/3T3 lines. Although some Friend gp71 interspecies reactivity was discernible on normal murine cells, the principal reactivity was shown to be group specific. Fresh thymocytes from BALB/cJ (G(IX-)), C57BL/G(IX-), and 129/J (G(IX+)) mouse strains were also reactive with Friend gp71 antiserum, and this activity, as well as that of an antiserum prepared against purified AKR gp71, were also group specific. An activity discriminating G(IX+) from G(IX-) thymocytes was not observed with either Friend or AKR gp71 antisera.

Original languageEnglish (US)
Pages (from-to)931-938
Number of pages8
JournalCancer Research
Volume37
Issue number3
StatePublished - 1977

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Oncogenic Viruses
Immune Sera
Glycoproteins
Thymocytes
Friend murine leukemia virus
Cell Line
Murine Leukemia Viruses
Fluorescent Antibody Technique
Genotype
Cell Membrane
Rabbits

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Immunofluorescent analysis of expression of the RNAT tumor virus major glycoprotein, gp71, on the surfaces of normal murine cells. / Cloyd, M. W.; Bolognesi, D. P.; Bigner, D. D.

In: Cancer Research, Vol. 37, No. 3, 1977, p. 931-938.

Research output: Contribution to journalArticle

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abstract = "The expression of the major glycoprotein, gp71, of murine leukemia virus was studied on the surfaces of a variety of normal murine cell lines with a monospecific rabbit antiserum raised against purified Friend murine leukemia virus gp71. Using viable cell membrane immunofluorescence, most established and early passage normal murine cell lines were significantly reactive with the antiserum, irrespective of neoplastic transformation, strain genotype, or whether they were of embryonic or adult tissue origin. The only murine cells tested not detectably expressing gp71 determinants were BALB/3T3 lines. Although some Friend gp71 interspecies reactivity was discernible on normal murine cells, the principal reactivity was shown to be group specific. Fresh thymocytes from BALB/cJ (G(IX-)), C57BL/G(IX-), and 129/J (G(IX+)) mouse strains were also reactive with Friend gp71 antiserum, and this activity, as well as that of an antiserum prepared against purified AKR gp71, were also group specific. An activity discriminating G(IX+) from G(IX-) thymocytes was not observed with either Friend or AKR gp71 antisera.",
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N2 - The expression of the major glycoprotein, gp71, of murine leukemia virus was studied on the surfaces of a variety of normal murine cell lines with a monospecific rabbit antiserum raised against purified Friend murine leukemia virus gp71. Using viable cell membrane immunofluorescence, most established and early passage normal murine cell lines were significantly reactive with the antiserum, irrespective of neoplastic transformation, strain genotype, or whether they were of embryonic or adult tissue origin. The only murine cells tested not detectably expressing gp71 determinants were BALB/3T3 lines. Although some Friend gp71 interspecies reactivity was discernible on normal murine cells, the principal reactivity was shown to be group specific. Fresh thymocytes from BALB/cJ (G(IX-)), C57BL/G(IX-), and 129/J (G(IX+)) mouse strains were also reactive with Friend gp71 antiserum, and this activity, as well as that of an antiserum prepared against purified AKR gp71, were also group specific. An activity discriminating G(IX+) from G(IX-) thymocytes was not observed with either Friend or AKR gp71 antisera.

AB - The expression of the major glycoprotein, gp71, of murine leukemia virus was studied on the surfaces of a variety of normal murine cell lines with a monospecific rabbit antiserum raised against purified Friend murine leukemia virus gp71. Using viable cell membrane immunofluorescence, most established and early passage normal murine cell lines were significantly reactive with the antiserum, irrespective of neoplastic transformation, strain genotype, or whether they were of embryonic or adult tissue origin. The only murine cells tested not detectably expressing gp71 determinants were BALB/3T3 lines. Although some Friend gp71 interspecies reactivity was discernible on normal murine cells, the principal reactivity was shown to be group specific. Fresh thymocytes from BALB/cJ (G(IX-)), C57BL/G(IX-), and 129/J (G(IX+)) mouse strains were also reactive with Friend gp71 antiserum, and this activity, as well as that of an antiserum prepared against purified AKR gp71, were also group specific. An activity discriminating G(IX+) from G(IX-) thymocytes was not observed with either Friend or AKR gp71 antisera.

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