Immunogenicity of a multicomponent DNA vaccine against visceral leishmaniasis in dogs

Omar A. Saldarriaga, Bruno Travi, Won Park, Luis E. Perez, Peter Melby

Research output: Contribution to journalArticle

34 Scopus citations

Abstract

Vaccination of dogs, the domestic reservoir of Leishmania chagasi, could not only decrease the burden of canine visceral leishmaniasis (VL), but could also indirectly reduce the incidence of human VL. Intramuscular vaccination of foxhounds with a Leishmania multicomponent (10 antigen) DNA vaccine resulted in antigen-induced lymphoproliferative and IFN-γ (but not IL-4) responses. This response was not augmented by co-administration of canine IL-12 or GM-CSF DNA adjuvants. The multicomponent DNA vaccine also induced a delayed type hypersensitivity (DTH) response to viable L. donovani promastigotes and led to a reduction of parasite burden in an in vitro intracellular infection model, and in the draining lymph node of dogs early after cutaneous challenge. Thus, the multicomponent DNA vaccine was effective in priming dogs for a parasite-specific type 1 cellular immune response, which was able to restrict parasite growth.

Original languageEnglish
Pages (from-to)1928-1940
Number of pages13
JournalVaccine
Volume24
Issue number11
DOIs
StatePublished - Mar 10 2006
Externally publishedYes

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Keywords

  • Cellular immunity
  • Interferon-gamma
  • Leishmania chagasi
  • Leishmania donovani
  • Vaccine
  • Visceral leishmaniasis

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Virology
  • Infectious Diseases
  • Public Health, Environmental and Occupational Health
  • veterinary(all)

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