Immunogenicity of experimental live attenuated Japanese encephalitis vaccine viruses and comparison with wild-type strains using monoclonal and polyclonal antibodies

B. K. Sil, M. R. Wills, J. X. Cao, R. Sharda, M. A. Islam, D. J. Stagg, A. D. Jennings, C. A. Gibson, A. D.T. Barrett

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

A total of 105 hybridomas secreting anti-Japanese encephalitis (JE) virus monoclonal antibodies (mAbs) were generated from six fusions against four strains of JE virus: wild-type strains SA14 and G8924 and live attenuated vaccines SA14-5-3 and SA14-14-2 (PDK-9). Most of the mAbs (87%) elicited haemagglutination inhibition activity while only a minority (24%) elicited neutralization. None of the mAbs prepared against SA14-5-3, parent of SA14-14-2, elicited neutralization while the only mAbs prepared against SA14-14-2 that elicited neutralization recognized flavivirus cross-reactive epitopes. In comparison, mAbs raised against wild-type strains showed that a spectrum of epitopes with different specificities, including JE type-specific epitopes, elicited neutralizing activity. Two mAbs, prepared against SA14-5-3 virus, were found to be vaccine-specific and five, prepared against strains SA14 and G8924, were wild-type-specific.

Original languageEnglish (US)
Pages (from-to)329-333
Number of pages5
JournalVaccine
Volume10
Issue number5
DOIs
StatePublished - 1992
Externally publishedYes

Keywords

  • Japanese encephalitis
  • flavivirus
  • monoclonal antibodies
  • vaccine

ASJC Scopus subject areas

  • Molecular Medicine
  • Immunology and Microbiology(all)
  • veterinary(all)
  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

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