Immunogenicity of Ra1A and its tissue-specific expression in hepatocellular carcinoma

K. Wang, Y. Chen, S. Liu, S. Qiu, S. Gao, X. Huang, J. Zhang, X. Peng, W. Qiani, Jian Ying Zhang

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

In order to understand the immunogenicity of a tumor-associated antigen (TAA), Ras family small GTP binding protein (Ra1A) in hepatocellular carcinoma (HCC), autoantibody responses to RalA were evaluated by enzyme-linked immunosorbent assay (ELISA), Western blotting and indirect immunofluorescence assay in sera from patients with HCC and sera from normal individuals. Immunohistochemistry (IHC) study with tissue array slides was also preformed to analyze protein expression profiles of RalA in HCC and control tissues. This study demonstrated that RalA had a relative higher frequency of autoantibody response in HCC (20.1%) compared to liver cirrhosis (3.3%), chronic hepatitis (0%), and normal individuals sera (0%). RalA also showed a stepwise increased expression from normal liver tissues (26.7%), liver cirrhosis tissues (45.0%) to HCC tissues (63.3%). Sensitivity and specificity of anti-RalA antibody in detection of HCC was 20.1% and 99.3%, respectively. The data suggested that RalA might contribute to liver malignant transformation, and could be used as a potential tumor marker in HCC detection.

Original languageEnglish (US)
Pages (from-to)735-743
Number of pages9
JournalInternational Journal of Immunopathology and Pharmacology
Volume22
Issue number3
DOIs
StatePublished - 2009

Keywords

  • Hepatocellular carcinoma
  • Immunoreactivity
  • Ra1A
  • Tissue expression
  • Tumor-associated antigen

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Pharmacology

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