Immunoglobulin G enzyme-linked immunosorbent assay using truncated nucleoproteins of Reston Ebola virus

Tetsuro Ikegami, M. Saijo, M. Niikura, M. E. Miranda, A. B. Calaor, M. Hernandez, D. L. Manalo, I. Kurane, Y. Yoshikawa, S. Morikawa

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Abstract

We developed an immunoglobulin G (IgG) enzyme-linked immunosorbent assay (ELISA), using partial recombinant nucleoproteins (rNP) of Reston Ebola virus (EBO-R) and Zaire Ebola virus (EBO-Z). We examined the reaction of 10 sera from cynomolgus macaques naturally infected with EBO-R to each of the partial rNP in the IgG ELISA. All the sera reacted to the C-terminal halves of the rNP of both EBO-R and EBO-Z. Most of the sera reacted to the RΔC (amino acid (aa) 360-739), and RΔ6 (aa 451-551) and/or RΔ8 (aa 631-739) at a higher dilution than to the corresponding truncated rNPs of EBO-Z. The results indicate that this IgG ELISA is useful for detecting EBO-R specific antibody, and may have a potential to discriminate EBO-R infection from other subtypes.

Original languageEnglish (US)
Pages (from-to)533-539
Number of pages7
JournalEpidemiology and Infection
Volume130
Issue number3
StatePublished - Jun 2003
Externally publishedYes

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Ebolavirus
Nucleoproteins
Immunoglobulin G
Enzyme-Linked Immunosorbent Assay
Amino Acids
Serum
Macaca
Antibodies
Infection

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Immunology

Cite this

Ikegami, T., Saijo, M., Niikura, M., Miranda, M. E., Calaor, A. B., Hernandez, M., ... Morikawa, S. (2003). Immunoglobulin G enzyme-linked immunosorbent assay using truncated nucleoproteins of Reston Ebola virus. Epidemiology and Infection, 130(3), 533-539.

Immunoglobulin G enzyme-linked immunosorbent assay using truncated nucleoproteins of Reston Ebola virus. / Ikegami, Tetsuro; Saijo, M.; Niikura, M.; Miranda, M. E.; Calaor, A. B.; Hernandez, M.; Manalo, D. L.; Kurane, I.; Yoshikawa, Y.; Morikawa, S.

In: Epidemiology and Infection, Vol. 130, No. 3, 06.2003, p. 533-539.

Research output: Contribution to journalArticle

Ikegami, T, Saijo, M, Niikura, M, Miranda, ME, Calaor, AB, Hernandez, M, Manalo, DL, Kurane, I, Yoshikawa, Y & Morikawa, S 2003, 'Immunoglobulin G enzyme-linked immunosorbent assay using truncated nucleoproteins of Reston Ebola virus', Epidemiology and Infection, vol. 130, no. 3, pp. 533-539.
Ikegami T, Saijo M, Niikura M, Miranda ME, Calaor AB, Hernandez M et al. Immunoglobulin G enzyme-linked immunosorbent assay using truncated nucleoproteins of Reston Ebola virus. Epidemiology and Infection. 2003 Jun;130(3):533-539.
Ikegami, Tetsuro ; Saijo, M. ; Niikura, M. ; Miranda, M. E. ; Calaor, A. B. ; Hernandez, M. ; Manalo, D. L. ; Kurane, I. ; Yoshikawa, Y. ; Morikawa, S. / Immunoglobulin G enzyme-linked immunosorbent assay using truncated nucleoproteins of Reston Ebola virus. In: Epidemiology and Infection. 2003 ; Vol. 130, No. 3. pp. 533-539.
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