Immunoglobulin variable region usage in human intestinal B lymphocytes

Robert P. McCabe, William L. Carroll, Maureen Egan, Steven M. Cohn, Marion Peters

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

The B cell repertoire was studied in intestinal mononuclear cells from normal individuals and patients with inflammatory bowel disease (IBD) by examining Ig heavy chain variable gene (V(H)) usage. Using reverse transcription of intestinal mucosal RNA followed by polymerase chain reaction with primers specific for each V(H) family and a housekeeping gene, a semiquantitative assay of V(H) family content in RNA samples was developed. While all V(H) family members were expressed, differences in V(H) usage in lamina propria intestinal B cells were noted between Crohn's disease, ulcerative colitis, and normal individuals. mRNA transcripts for V(H)4 were present at seemingly higher levels than their genomic representation and transcripts for V(H)4 and V(H)4 appeared to have higher levels in active, compared to inactive, IBD. Thus, within the massive polyclonal intestinal B cell response, there is a skewed V(H) usage which may be relevant to the antigenic and/or autoimmune response noted in IBD.

Original languageEnglish (US)
Pages (from-to)240-245
Number of pages6
JournalClinical Immunology and Immunopathology
Volume71
Issue number2
DOIs
StatePublished - May 1994

ASJC Scopus subject areas

  • Immunology and Allergy
  • Pathology and Forensic Medicine
  • Immunology

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