Immunohistochemical localization of protein 3-nitrotyrosine and S- nitrosocysteine in a murine model of inhaled nitric oxide therapy

Scott A. Lorch, Raymond Foust, Andrew Gow, Mark Arkovitz, Andrew L. Salzman, Csaba Szabo, Bernard Vayert, Michel Geffard, Harry Ischiropoulos

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Inhaled nitric oxide (INO) therapy is currently used clinically to selectively dilate the pulmonary vasculature and to help treat persistent pulmonary hypertension and bronchopulmonary dysplasia in the neonate. However, in the presence of oxygen or superoxide, nitric oxide forms potentially harmful reactive nitrogen species. Using an experimental mice model, we examined the effects of concurrent hyperoxia and INO on protein tyrosine nitration and cysteine S-nitrosylation in pulmonary tissue. Data showed enhanced 3-nitrotyrosine staining within the airway epithelium and alveolar interstitium of mice lungs treated with hyperoxia, which did not increase significantly with INO administration. Within the alveolar interstitium, 3-nitrotyrosine staining was localized to macrophages. S- Nitrosocysteine staining in airway epithelium was significantly enhanced with INO administration regardless of oxygen content. These data suggest that the formation of protein S-nitrosocysteine is the major protein modification during administration of INO.

Original languageEnglish (US)
Pages (from-to)798-805
Number of pages8
JournalPediatric Research
Volume47
Issue number6
StatePublished - Jun 2000
Externally publishedYes

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Nitric Oxide
Hyperoxia
Proteins
Staining and Labeling
Lung
Therapeutics
Epithelium
Oxygen
Reactive Nitrogen Species
Bronchopulmonary Dysplasia
Protein S
Pulmonary Hypertension
Superoxides
Cysteine
Tyrosine
3-nitrotyrosine
S-nitrosocysteine
Theoretical Models
Macrophages

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health

Cite this

Lorch, S. A., Foust, R., Gow, A., Arkovitz, M., Salzman, A. L., Szabo, C., ... Ischiropoulos, H. (2000). Immunohistochemical localization of protein 3-nitrotyrosine and S- nitrosocysteine in a murine model of inhaled nitric oxide therapy. Pediatric Research, 47(6), 798-805.

Immunohistochemical localization of protein 3-nitrotyrosine and S- nitrosocysteine in a murine model of inhaled nitric oxide therapy. / Lorch, Scott A.; Foust, Raymond; Gow, Andrew; Arkovitz, Mark; Salzman, Andrew L.; Szabo, Csaba; Vayert, Bernard; Geffard, Michel; Ischiropoulos, Harry.

In: Pediatric Research, Vol. 47, No. 6, 06.2000, p. 798-805.

Research output: Contribution to journalArticle

Lorch, SA, Foust, R, Gow, A, Arkovitz, M, Salzman, AL, Szabo, C, Vayert, B, Geffard, M & Ischiropoulos, H 2000, 'Immunohistochemical localization of protein 3-nitrotyrosine and S- nitrosocysteine in a murine model of inhaled nitric oxide therapy', Pediatric Research, vol. 47, no. 6, pp. 798-805.
Lorch, Scott A. ; Foust, Raymond ; Gow, Andrew ; Arkovitz, Mark ; Salzman, Andrew L. ; Szabo, Csaba ; Vayert, Bernard ; Geffard, Michel ; Ischiropoulos, Harry. / Immunohistochemical localization of protein 3-nitrotyrosine and S- nitrosocysteine in a murine model of inhaled nitric oxide therapy. In: Pediatric Research. 2000 ; Vol. 47, No. 6. pp. 798-805.
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AB - Inhaled nitric oxide (INO) therapy is currently used clinically to selectively dilate the pulmonary vasculature and to help treat persistent pulmonary hypertension and bronchopulmonary dysplasia in the neonate. However, in the presence of oxygen or superoxide, nitric oxide forms potentially harmful reactive nitrogen species. Using an experimental mice model, we examined the effects of concurrent hyperoxia and INO on protein tyrosine nitration and cysteine S-nitrosylation in pulmonary tissue. Data showed enhanced 3-nitrotyrosine staining within the airway epithelium and alveolar interstitium of mice lungs treated with hyperoxia, which did not increase significantly with INO administration. Within the alveolar interstitium, 3-nitrotyrosine staining was localized to macrophages. S- Nitrosocysteine staining in airway epithelium was significantly enhanced with INO administration regardless of oxygen content. These data suggest that the formation of protein S-nitrosocysteine is the major protein modification during administration of INO.

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