Immunohistochemical localization of seven different peptides in the human spinal cord

K. Chung, R. P. Briner, S. M. Carlton, K. N. Westlund

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

It is necessary to study the normal chemical contents in the human spinal cord in order to understand neurochemical changes that might occur under pathological conditions. In the present study, the comparative distribution of seven peptides was examined immunohistochemically in four levels (cervical C; thoracic, T; lumbar, L; sacral, S) of the human spinal cord by means of the peroxidase-antiperoxidase technique. The peptides examined incluced bombesin (BOM), substance P (SP), cholecystokinin (CCK), somatostatin (SOM), methionine-enkephalin (M-ENK), vasoactive intestinal polypeptide (VIP), and thyrotropin releasing hormone (TRH). Among the seven peptides examined, four (BOM, CCK, SOM, and TRH) have never been described in the human spinal cord and the present work clearly demonstrates their existence in specific patterns. The terminals that were immunostained for BOM and CCK were localized in high concentration in the superficial dorsal horn (laminae I-II) in moderate amounts in the lateral part of laminae V and VII, and lesser amounts in the intermediate gray (lamina VII) and the dorsal part of the central gray (lamina X). Whereas BOM showed a similar distribution pattern at all spinal levels, CCK was mainly found in thoracic and lumbar levels. The SOM terminals were localized in the superficial dorsal horn (the highest density in lamina II but very few in lamina I), the intermediolateral cell column, intermediated gray, and central gray. This peptide was more widely distributed in the sacral cord with its terminal field extending into the ventral horn. The TRH terminals were mainly located in the ventral horn. Frequently, TRH terminals were seen adjacent to large ventral horn neurons. Furthermore, many neurons in the ventral and intermediate gray and Clarke's column demonstrated TRH immunoreactivity. The other three peptides (SP, M-ENK, and VIP) have been previously demonstrated in the human spinal cord and the present study confirmed their general spinal distribution with minor differences.

Original languageEnglish (US)
Pages (from-to)158-170
Number of pages13
JournalJournal of Comparative Neurology
Volume280
Issue number1
StatePublished - 1989

Fingerprint

Thyrotropin-Releasing Hormone
Bombesin
Cholecystokinin
Spinal Cord
Peptides
Somatostatin
Methionine Enkephalin
Vasoactive Intestinal Peptide
Horns
Substance P
Thorax
Substantia Gelatinosa
Anterior Horn Cells
Peroxidase
Spinal Cord Dorsal Horn
Neurons

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Chung, K., Briner, R. P., Carlton, S. M., & Westlund, K. N. (1989). Immunohistochemical localization of seven different peptides in the human spinal cord. Journal of Comparative Neurology, 280(1), 158-170.

Immunohistochemical localization of seven different peptides in the human spinal cord. / Chung, K.; Briner, R. P.; Carlton, S. M.; Westlund, K. N.

In: Journal of Comparative Neurology, Vol. 280, No. 1, 1989, p. 158-170.

Research output: Contribution to journalArticle

Chung, K, Briner, RP, Carlton, SM & Westlund, KN 1989, 'Immunohistochemical localization of seven different peptides in the human spinal cord', Journal of Comparative Neurology, vol. 280, no. 1, pp. 158-170.
Chung, K. ; Briner, R. P. ; Carlton, S. M. ; Westlund, K. N. / Immunohistochemical localization of seven different peptides in the human spinal cord. In: Journal of Comparative Neurology. 1989 ; Vol. 280, No. 1. pp. 158-170.
@article{4e67a0ec963043eea66a8a1a0ecc3ddb,
title = "Immunohistochemical localization of seven different peptides in the human spinal cord",
abstract = "It is necessary to study the normal chemical contents in the human spinal cord in order to understand neurochemical changes that might occur under pathological conditions. In the present study, the comparative distribution of seven peptides was examined immunohistochemically in four levels (cervical C; thoracic, T; lumbar, L; sacral, S) of the human spinal cord by means of the peroxidase-antiperoxidase technique. The peptides examined incluced bombesin (BOM), substance P (SP), cholecystokinin (CCK), somatostatin (SOM), methionine-enkephalin (M-ENK), vasoactive intestinal polypeptide (VIP), and thyrotropin releasing hormone (TRH). Among the seven peptides examined, four (BOM, CCK, SOM, and TRH) have never been described in the human spinal cord and the present work clearly demonstrates their existence in specific patterns. The terminals that were immunostained for BOM and CCK were localized in high concentration in the superficial dorsal horn (laminae I-II) in moderate amounts in the lateral part of laminae V and VII, and lesser amounts in the intermediate gray (lamina VII) and the dorsal part of the central gray (lamina X). Whereas BOM showed a similar distribution pattern at all spinal levels, CCK was mainly found in thoracic and lumbar levels. The SOM terminals were localized in the superficial dorsal horn (the highest density in lamina II but very few in lamina I), the intermediolateral cell column, intermediated gray, and central gray. This peptide was more widely distributed in the sacral cord with its terminal field extending into the ventral horn. The TRH terminals were mainly located in the ventral horn. Frequently, TRH terminals were seen adjacent to large ventral horn neurons. Furthermore, many neurons in the ventral and intermediate gray and Clarke's column demonstrated TRH immunoreactivity. The other three peptides (SP, M-ENK, and VIP) have been previously demonstrated in the human spinal cord and the present study confirmed their general spinal distribution with minor differences.",
author = "K. Chung and Briner, {R. P.} and Carlton, {S. M.} and Westlund, {K. N.}",
year = "1989",
language = "English (US)",
volume = "280",
pages = "158--170",
journal = "Journal of Comparative Neurology",
issn = "0021-9967",
publisher = "Wiley-Liss Inc.",
number = "1",

}

TY - JOUR

T1 - Immunohistochemical localization of seven different peptides in the human spinal cord

AU - Chung, K.

AU - Briner, R. P.

AU - Carlton, S. M.

AU - Westlund, K. N.

PY - 1989

Y1 - 1989

N2 - It is necessary to study the normal chemical contents in the human spinal cord in order to understand neurochemical changes that might occur under pathological conditions. In the present study, the comparative distribution of seven peptides was examined immunohistochemically in four levels (cervical C; thoracic, T; lumbar, L; sacral, S) of the human spinal cord by means of the peroxidase-antiperoxidase technique. The peptides examined incluced bombesin (BOM), substance P (SP), cholecystokinin (CCK), somatostatin (SOM), methionine-enkephalin (M-ENK), vasoactive intestinal polypeptide (VIP), and thyrotropin releasing hormone (TRH). Among the seven peptides examined, four (BOM, CCK, SOM, and TRH) have never been described in the human spinal cord and the present work clearly demonstrates their existence in specific patterns. The terminals that were immunostained for BOM and CCK were localized in high concentration in the superficial dorsal horn (laminae I-II) in moderate amounts in the lateral part of laminae V and VII, and lesser amounts in the intermediate gray (lamina VII) and the dorsal part of the central gray (lamina X). Whereas BOM showed a similar distribution pattern at all spinal levels, CCK was mainly found in thoracic and lumbar levels. The SOM terminals were localized in the superficial dorsal horn (the highest density in lamina II but very few in lamina I), the intermediolateral cell column, intermediated gray, and central gray. This peptide was more widely distributed in the sacral cord with its terminal field extending into the ventral horn. The TRH terminals were mainly located in the ventral horn. Frequently, TRH terminals were seen adjacent to large ventral horn neurons. Furthermore, many neurons in the ventral and intermediate gray and Clarke's column demonstrated TRH immunoreactivity. The other three peptides (SP, M-ENK, and VIP) have been previously demonstrated in the human spinal cord and the present study confirmed their general spinal distribution with minor differences.

AB - It is necessary to study the normal chemical contents in the human spinal cord in order to understand neurochemical changes that might occur under pathological conditions. In the present study, the comparative distribution of seven peptides was examined immunohistochemically in four levels (cervical C; thoracic, T; lumbar, L; sacral, S) of the human spinal cord by means of the peroxidase-antiperoxidase technique. The peptides examined incluced bombesin (BOM), substance P (SP), cholecystokinin (CCK), somatostatin (SOM), methionine-enkephalin (M-ENK), vasoactive intestinal polypeptide (VIP), and thyrotropin releasing hormone (TRH). Among the seven peptides examined, four (BOM, CCK, SOM, and TRH) have never been described in the human spinal cord and the present work clearly demonstrates their existence in specific patterns. The terminals that were immunostained for BOM and CCK were localized in high concentration in the superficial dorsal horn (laminae I-II) in moderate amounts in the lateral part of laminae V and VII, and lesser amounts in the intermediate gray (lamina VII) and the dorsal part of the central gray (lamina X). Whereas BOM showed a similar distribution pattern at all spinal levels, CCK was mainly found in thoracic and lumbar levels. The SOM terminals were localized in the superficial dorsal horn (the highest density in lamina II but very few in lamina I), the intermediolateral cell column, intermediated gray, and central gray. This peptide was more widely distributed in the sacral cord with its terminal field extending into the ventral horn. The TRH terminals were mainly located in the ventral horn. Frequently, TRH terminals were seen adjacent to large ventral horn neurons. Furthermore, many neurons in the ventral and intermediate gray and Clarke's column demonstrated TRH immunoreactivity. The other three peptides (SP, M-ENK, and VIP) have been previously demonstrated in the human spinal cord and the present study confirmed their general spinal distribution with minor differences.

UR - http://www.scopus.com/inward/record.url?scp=0024520286&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024520286&partnerID=8YFLogxK

M3 - Article

C2 - 2563739

AN - SCOPUS:0024520286

VL - 280

SP - 158

EP - 170

JO - Journal of Comparative Neurology

JF - Journal of Comparative Neurology

SN - 0021-9967

IS - 1

ER -