Immunologic determinants of disease evolution in localized cutaneous leishmaniasis due to Leishmania major

Hechmi Louzir, Peter Melby, Afif Ben Salah, Héla Marrakchi, Karim Aoun, Riadh Ben Ismail, Koussay Dellagi

Research output: Contribution to journalArticle

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Abstract

Localized cutaneous leishmaniasis caused by Leishmania major is polymorphic in its clinical presentation and evolution. Clinical and parasitologic features and disease evolution of 112 Tunisian patients was evaluated. The expression of interleukin (IL)-4, IL-6, IL-10, IL-12 (p40), interferon (IFN)-γ, and tumor necrosis factor (TNF)-α mRNA was analyzed by reverse transcription-polymerase chain reaction in 73 biopsies. Cytokine mRNA expression varied individually over a wide range; TNF-α, IL-6, and IFN-γ were detectable in >90% of lesions, IL-12 and IL-10 in 40% and 70%, respectively, and IL-4 in only 9%. Statistical analysis demonstrated positive association between the level of IL-12 and IFN-γ and the presence of parasites in the lesions. Unfavorable evolution of the lesions was positively associated with high IL-10, IL-12, and IFN-γ mRNA expression. These results indicate that an unfavorable clinical outcome was not related to an inadequate Th1 cell response and suggest that the macrophage-activating effect of IFN-γ may be inhibited by the concomitant expression of IL-10.

Original languageEnglish (US)
Pages (from-to)1687-1695
Number of pages9
JournalJournal of Infectious Diseases
Volume177
Issue number6
StatePublished - 1998
Externally publishedYes

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Leishmania major
Cutaneous Leishmaniasis
Immune System Diseases
Interferons
Interleukin-12
Interleukin-10
Interleukin-4
Messenger RNA
Interleukin-6
Tumor Necrosis Factor-alpha
Th1 Cells
Reverse Transcription
Parasites
Macrophages
Cytokines
Biopsy
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Immunology

Cite this

Louzir, H., Melby, P., Salah, A. B., Marrakchi, H., Aoun, K., Ismail, R. B., & Dellagi, K. (1998). Immunologic determinants of disease evolution in localized cutaneous leishmaniasis due to Leishmania major. Journal of Infectious Diseases, 177(6), 1687-1695.

Immunologic determinants of disease evolution in localized cutaneous leishmaniasis due to Leishmania major. / Louzir, Hechmi; Melby, Peter; Salah, Afif Ben; Marrakchi, Héla; Aoun, Karim; Ismail, Riadh Ben; Dellagi, Koussay.

In: Journal of Infectious Diseases, Vol. 177, No. 6, 1998, p. 1687-1695.

Research output: Contribution to journalArticle

Louzir, H, Melby, P, Salah, AB, Marrakchi, H, Aoun, K, Ismail, RB & Dellagi, K 1998, 'Immunologic determinants of disease evolution in localized cutaneous leishmaniasis due to Leishmania major', Journal of Infectious Diseases, vol. 177, no. 6, pp. 1687-1695.
Louzir, Hechmi ; Melby, Peter ; Salah, Afif Ben ; Marrakchi, Héla ; Aoun, Karim ; Ismail, Riadh Ben ; Dellagi, Koussay. / Immunologic determinants of disease evolution in localized cutaneous leishmaniasis due to Leishmania major. In: Journal of Infectious Diseases. 1998 ; Vol. 177, No. 6. pp. 1687-1695.
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