Immunomodulatory effects of radiofrequency ablation in a breast cancer model

Valentina K. Todorova, Vicki Klimberg, Leah Hennings, Thomas Kieber-Emmons, Anastas Pashov

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Radiofrequency ablation (RFA), a minimally invasive surgical procedure has an increasing application in the surgical treatment of tumors. Data indicate that RFA might stimulate anti-cancer immunity possibly through the induction of necrosis and heat shock proteins (HSP) expression. This study tests a hypothesis that RFA leads to bidirectional immunoregulation. Experimental rat breast tumors were treated with RFA, surgical excision or sham operation. RFA resulted in the highest NK cells infiltration, increased HSP70 expression and activation of caspase-3 enzyme in the tumor margins. A significant reduction of the circulatory regulatory T (Treg) cells was found in both RFA- and excision-treated rats, although less pronounced in the RFA-group. The splenocyte proliferation to tumor cell lysate was stronger in the RFA-treated rats in comparison with untreated tumor-bearing rats. The potential role of self-HSP for immunomodulation was examined using in vitro proliferation assay to tetanus toxoid using human peripheral leukocytes. The response to the tetanus toxoid was significantly suppressed by HSP90 plus auto-antibodies versus HSP90 or auto-antibodies alone. In conclusion, RFA reduced the circulatory Tregs although not as efficient as tumor excision. HSPs plus natural antibodies suppress the anti-tumor response probably by stimulating Tregs. Therefore, RFA may play a role in anti-cancer therapy if combined with Tregs suppression.

Original languageEnglish (US)
Pages (from-to)74-92
Number of pages19
JournalImmunological Investigations
Volume39
Issue number1
DOIs
StatePublished - 2010
Externally publishedYes

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Breast Neoplasms
Neoplasms
Tetanus Toxoid
Regulatory T-Lymphocytes
Heat-Shock Proteins
Minimally Invasive Surgical Procedures
Immunomodulation
Antibodies
Caspase 3
Natural Killer Cells
Anti-Idiotypic Antibodies
Immunity
Leukocytes
Necrosis
Enzymes
Therapeutics

Keywords

  • Immunity
  • Natural killer (NK) cells
  • Radiofrequency ablation (RFA)
  • Regulatory T (Treg) cells

ASJC Scopus subject areas

  • Immunology

Cite this

Immunomodulatory effects of radiofrequency ablation in a breast cancer model. / Todorova, Valentina K.; Klimberg, Vicki; Hennings, Leah; Kieber-Emmons, Thomas; Pashov, Anastas.

In: Immunological Investigations, Vol. 39, No. 1, 2010, p. 74-92.

Research output: Contribution to journalArticle

Todorova, Valentina K. ; Klimberg, Vicki ; Hennings, Leah ; Kieber-Emmons, Thomas ; Pashov, Anastas. / Immunomodulatory effects of radiofrequency ablation in a breast cancer model. In: Immunological Investigations. 2010 ; Vol. 39, No. 1. pp. 74-92.
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