Immunoreactive prohormone atrial natriuretic peptides 1-30 and 31-67: Existence of a single circulating amino-terminal peptide

Yu Ming Chen, Peggy A. Whitson, Nitza M. Cintrón

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

Sep-Pak C18 extraction of human plasma and radioimmunoassay using antibodies which recognize atrial natriuretic peptide (99-128) and the prohormone sequences 1-30 and 31-67 resulted in mean values from 20 normal subjects of 26.2 (±9.2), 362 (±173) and 368 (±160) pg/ml, respectively. A high correlation coefficient between values obtained using antibodies recognizing prohormone sequences 1-30 and 31-67 was observed (R=0.84). Extracted plasma immunoreactivity of 1-30 and 31-67 both eluted at 46% acetonitrile. In contrast, chromatographic elution of synthetic peptides 1-30 and 31-67 was observed at 48 and 39% acetonitrile, respectively. Data suggest that the radioimmunoassay of plasma using antibodies recognizing prohormone sequences 1-30 and 31-67 may represent the measurement of a unique larger amino-terminal peptide fragment containing antigenic sites recognized by both antisera.

Original languageEnglish (US)
Pages (from-to)794-800
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume166
Issue number2
DOIs
StatePublished - Jan 30 1990
Externally publishedYes

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ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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