Immunostimulatory activity of potential probiotic yeast strains in the dorsal air pouch system and the gut mucosa

A. Kourelis, C. Kotzamanidis, E. Litopoulou-Tzanetaki, John Papaconstantinou, N. Tzanetakis, M. Yiangou

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Aims: To determine the immunostimulatory activity of 15 presumptive probiotic yeast strains in the dorsal air pouch system in comparison with their activity in the gut mucosa. Methods and Results: Presumptive probiotic yeast strains previously isolated from human gastrointestinal tract and Feta cheese were further characterized genotypically and biochemically. The Saccharomyces cerevisiae 982, Saccharomyces boulardii KK1 and Kluyveromyces lactis 630 strains exhibited in the air pouch increased polymorphonuclear cell influx and phagocytic activity as well as cytokine production with similar potency as the probiotics Ultra levure S. boulardii and Lactobacillus acidophilus NCFB 1748. Oral administration of these strains in mice results in differential activation of small intestine immune responses concerning IgA and cytokine production as well as Toll-like receptor expression. Conclusion: Besides the Saccharomyces strains 982 and KK1, the K. lactis 630 strain could also be considered as a candidate probiotic. Significance and Impact of the Study: The air pouch model may be used as an alternative and rapid method for the discrimination and selection of potential probiotic yeast strains.

Original languageEnglish (US)
Pages (from-to)260-271
Number of pages12
JournalJournal of Applied Microbiology
Volume109
Issue number1
DOIs
StatePublished - Jul 2010

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Probiotics
Mucous Membrane
Yeasts
Air
Cytokines
Kluyveromyces
Lactobacillus acidophilus
Saccharomyces
Toll-Like Receptors
Cheese
Phagocytes
Immunoglobulin A
Small Intestine
Oral Administration
Saccharomyces cerevisiae
Gastrointestinal Tract
Saccharomyces boulardii

Keywords

  • Air pouch
  • Cytokines
  • Gut mucosa
  • Probiotic
  • Yeast

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Biotechnology

Cite this

Immunostimulatory activity of potential probiotic yeast strains in the dorsal air pouch system and the gut mucosa. / Kourelis, A.; Kotzamanidis, C.; Litopoulou-Tzanetaki, E.; Papaconstantinou, John; Tzanetakis, N.; Yiangou, M.

In: Journal of Applied Microbiology, Vol. 109, No. 1, 07.2010, p. 260-271.

Research output: Contribution to journalArticle

Kourelis, A. ; Kotzamanidis, C. ; Litopoulou-Tzanetaki, E. ; Papaconstantinou, John ; Tzanetakis, N. ; Yiangou, M. / Immunostimulatory activity of potential probiotic yeast strains in the dorsal air pouch system and the gut mucosa. In: Journal of Applied Microbiology. 2010 ; Vol. 109, No. 1. pp. 260-271.
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