Impaired integrity of DNA after recovery from inflammation causes persistent dysfunction of colonic smooth muscle

Kuicheon Choi, Jinghong Chen, Sankar Mitra, Sushil K. Sarna

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Background & Aims: Patients with inflammatory bowel disease who are in remission and those who developed irritable bowel syndrome after enteric infection continue to have symptoms of diarrhea or constipation in the absence of overt inflammation, indicating motility dysfunction. We investigated whether oxidative stress during inflammation impairs integrity of the promoter of Cacna1c, which encodes the pore-forming α 1C subunit of Ca v1.2b calcium channels. Methods: We used long-extension polymerase chain reaction to evaluate DNA integrity in tissues from distal colons of rats; trinitrobenzene sulfonic acid was used to induce inflammation. Results: The H 2O 2 increased in the muscularis externa 17 days after inflammation was induced with trinitrobenzene sulfonic acid. The oxidative stress significantly impaired DNA integrity in 2 specific segments of the Cacna1c promoter: -506 to -260 and -2193 to -1542. The impairment peaked at day 3 and recovered partially by day 7 after induction of inflammation; expression of the products of Cacna1c followed a similar time course. Oxidative stress suppressed the expression of nuclear factor-erythroid-2related factor 2 (Nrf2), an important regulator of anti-oxidant proteins. Intraperitoneal administration of sulforaphane significantly reversed the suppression of Nrf2, oxidative damage in the promoter of Cacna1c, and suppression of Cacna1c on day 7 of inflammation. The inflammation subsided completely by 56 days after inflammation was induced; however, impairment of DNA integrity, expression of Nrf2 and Cacna1c, and smooth muscle reactivity to acetylcholine remained suppressed at this time point. Conclusions: Oxidative stress during inflammation impairs the integrity of the promoter of Cacna1c; impairment persists partially after inflammation has subsided. Reduced transcription of Cacna1c contributes to smooth muscle dysfunction in the absence of inflammation.

Original languageEnglish (US)
JournalGastroenterology
Volume141
Issue number4
DOIs
StatePublished - Oct 2011

Fingerprint

Smooth Muscle
Inflammation
DNA
Oxidative Stress
Trinitrobenzenes
Sulfonic Acids
Irritable Bowel Syndrome
Constipation
Calcium Channels
Inflammatory Bowel Diseases
Oxidants
Acetylcholine
Diarrhea
Colon
Polymerase Chain Reaction
Infection

Keywords

  • Damage
  • DNA Repair
  • Gastrointestinal Inflammation
  • IBD
  • IBS
  • Mutation

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Impaired integrity of DNA after recovery from inflammation causes persistent dysfunction of colonic smooth muscle. / Choi, Kuicheon; Chen, Jinghong; Mitra, Sankar; Sarna, Sushil K.

In: Gastroenterology, Vol. 141, No. 4, 10.2011.

Research output: Contribution to journalArticle

Choi, Kuicheon ; Chen, Jinghong ; Mitra, Sankar ; Sarna, Sushil K. / Impaired integrity of DNA after recovery from inflammation causes persistent dysfunction of colonic smooth muscle. In: Gastroenterology. 2011 ; Vol. 141, No. 4.
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abstract = "Background & Aims: Patients with inflammatory bowel disease who are in remission and those who developed irritable bowel syndrome after enteric infection continue to have symptoms of diarrhea or constipation in the absence of overt inflammation, indicating motility dysfunction. We investigated whether oxidative stress during inflammation impairs integrity of the promoter of Cacna1c, which encodes the pore-forming α 1C subunit of Ca v1.2b calcium channels. Methods: We used long-extension polymerase chain reaction to evaluate DNA integrity in tissues from distal colons of rats; trinitrobenzene sulfonic acid was used to induce inflammation. Results: The H 2O 2 increased in the muscularis externa 17 days after inflammation was induced with trinitrobenzene sulfonic acid. The oxidative stress significantly impaired DNA integrity in 2 specific segments of the Cacna1c promoter: -506 to -260 and -2193 to -1542. The impairment peaked at day 3 and recovered partially by day 7 after induction of inflammation; expression of the products of Cacna1c followed a similar time course. Oxidative stress suppressed the expression of nuclear factor-erythroid-2related factor 2 (Nrf2), an important regulator of anti-oxidant proteins. Intraperitoneal administration of sulforaphane significantly reversed the suppression of Nrf2, oxidative damage in the promoter of Cacna1c, and suppression of Cacna1c on day 7 of inflammation. The inflammation subsided completely by 56 days after inflammation was induced; however, impairment of DNA integrity, expression of Nrf2 and Cacna1c, and smooth muscle reactivity to acetylcholine remained suppressed at this time point. Conclusions: Oxidative stress during inflammation impairs the integrity of the promoter of Cacna1c; impairment persists partially after inflammation has subsided. Reduced transcription of Cacna1c contributes to smooth muscle dysfunction in the absence of inflammation.",
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