Impaired T-cell activation in patients with systemic lupus erythematosus

Stanislaw Sierakowski, Eugene J. Kucharz, Robert W. Lightfoot, James S. Goodwin

Research output: Contribution to journalArticle

33 Scopus citations

Abstract

Interleukin-2 (IL-2) production was studied in T lymphocytes from 32 patients with systemic lupus erythematosus (SLE) and 27 healthy volunteers. The IL-2 production by phytohemagglutinin (PHA)-stimulated cells from SLE patients was significantly depressed compared to control values, with a correlation between degree of depression and disease activity. The depressed IL-2 production by SLE T cells are largely reversed by the addition of either phorbol ester (PMA) or partially by a calcium ionophore. SLE T cells had significantly lower peak increases in intracellular free calcium ([Ca2+]i) than controls after stimulation by PHA or by a monoclonal antibody against the CD3 antigen. This abnormality was found even in T cells from patients with mild disease activity or in those whose T cells produced normal amounts of IL-2. Calcium ionophore produced similar increases in [Ca2+]i in SLE patients as in normals. These results suggest that a major component of the defect responsible for decreased IL-2 production by SLE lymphocytes is proximal to protein kinase C activation and may involve impaired signal transduction after activation of the antigen receptor complex.

Original languageEnglish (US)
Pages (from-to)469-476
Number of pages8
JournalJournal of Clinical Immunology
Volume9
Issue number6
DOIs
StatePublished - Nov 1 1989
Externally publishedYes

Keywords

  • Interleukin-2
  • T-cell activation
  • intracellular free calcium
  • systemic lupus erythematosus

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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