Imperatorin sensitizes anoikis and inhibits anchorage-independent growth of lung cancer cells

Kanuengnit Choochuay, Preedakorn Chunhacha, Varisa Pongrakhananon, Rataya Luechapudiporn, Pithi Chanvorachote

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

The anoikis-sensitization activity of imperatorin, an active furanocoumarin component of Angelica dahurica root, is reported herein for the first time. The present study demonstrated that the imperatorin treatment at sub-toxic concentrations enhanced human lung cancer H23 cell apoptosis after detachment. A Western blot analysis showed that imperatorin significantly enhanced the p53 protein level, which subsequently down-regulated Mcl-1 protein and up-regulated Bax, while it had a minimal effect on Bcl-2 expression. In addition, an anchorage-independent growth assay was performed to support the anti-metastasis potential of imperatorin. Consistent with anoikis assay, imperatorin exhibited a strong inhibitory effect on the anchorage-independent growth of the cells. Further, this study demonstrated that imperatorin sensitizes anoikis in other lung cancer cells, namely, H292 and A549. Because anoikis was shown to be a critical hindrance in preventing cancer cell metastasis, the knowledge regarding such an activity and an underlying mechanism may lead to the development of this compound for a cancer therapy.

Original languageEnglish (US)
Pages (from-to)599-606
Number of pages8
JournalJournal of Natural Medicines
Volume67
Issue number3
DOIs
StatePublished - Jul 2013
Externally publishedYes

Fingerprint

Anoikis
Lung Neoplasms
Cells
Growth
Assays
Angelica
Neoplasm Metastasis
Poisons
imperatorin
Neoplasms
Proteins
Western Blotting
Apoptosis

Keywords

  • Anchorage-independent growth
  • Anoikis
  • Imperatorin
  • Lung cancer cells

ASJC Scopus subject areas

  • Organic Chemistry
  • Drug Discovery
  • Pharmaceutical Science
  • Complementary and alternative medicine

Cite this

Choochuay, K., Chunhacha, P., Pongrakhananon, V., Luechapudiporn, R., & Chanvorachote, P. (2013). Imperatorin sensitizes anoikis and inhibits anchorage-independent growth of lung cancer cells. Journal of Natural Medicines, 67(3), 599-606. https://doi.org/10.1007/s11418-012-0719-y

Imperatorin sensitizes anoikis and inhibits anchorage-independent growth of lung cancer cells. / Choochuay, Kanuengnit; Chunhacha, Preedakorn; Pongrakhananon, Varisa; Luechapudiporn, Rataya; Chanvorachote, Pithi.

In: Journal of Natural Medicines, Vol. 67, No. 3, 07.2013, p. 599-606.

Research output: Contribution to journalArticle

Choochuay, K, Chunhacha, P, Pongrakhananon, V, Luechapudiporn, R & Chanvorachote, P 2013, 'Imperatorin sensitizes anoikis and inhibits anchorage-independent growth of lung cancer cells', Journal of Natural Medicines, vol. 67, no. 3, pp. 599-606. https://doi.org/10.1007/s11418-012-0719-y
Choochuay, Kanuengnit ; Chunhacha, Preedakorn ; Pongrakhananon, Varisa ; Luechapudiporn, Rataya ; Chanvorachote, Pithi. / Imperatorin sensitizes anoikis and inhibits anchorage-independent growth of lung cancer cells. In: Journal of Natural Medicines. 2013 ; Vol. 67, No. 3. pp. 599-606.
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