Importance of basal nitric oxide synthesis in regulation of myocardial blood flow

Zoltán Benyó, Gergely Kiss, Csaba Szabo, Csilla Csáki, Arisztid G B Kovách

Research output: Contribution to journalArticle

Abstract

Study objective - The aim was to investigate whether basal coronary vascular tone and myocardial perfusion depend upon endothelial nitric oxide (NO) synthesis. Design - Myocardial blood flow and vascular resistance of the left and right ventricles were studied before and after intravenous infusions of either NG-nitro-L-arginine (L-NA), a specific inhibitor of NO synthase, or L-arginine, the precursor of NO synthesis. Radiolabelled microspheres were used to study myocardial blood flow in small tissue sections. Experimental material - 14 anaesthetised male cats, weight 2.1-3.5 kg, were used. Measurements and main results - Measurements were made before and 15 and 40 min after L-NA treatment (30 mg·kg-1 bolus followed by 1 mg·kg-1·min-1 infusion; n=8), and before and 15 min after L-arginine treatment (30 mg·kg-1 bolus followed by 10 mg·kg-1·min-1 infusion; n=6). L-NA significantly reduced coronary blood flow to the left and right ventricle, by 30(SEM 9) and 48(6)% respectively, after 15 min, but only to the right ventricle, by 45(8)%, after 40 min. Mean arterial pressure and myocardial vascular resistance were raised during the L-NA infusion. In contrast, L-arginine did not elicit any change in the variables studied. Conclusions - The conductance of the coronary vascular bed and the resting myocardial blood flow is regulated by L-arginine derived nitric oxide, and exogenous L-arginine availability is not a limiting factor in this NO generation.

Original languageEnglish (US)
Pages (from-to)700-703
Number of pages4
JournalCardiovascular Research
Volume25
Issue number8
StatePublished - 1991
Externally publishedYes

Fingerprint

Nitric Oxide
Arginine
Nitric oxide
Blood Flow
Heart Ventricles
Blood
Synthesis
Vascular Resistance
Blood Vessels
Coronary Blood Flow
Microspheres
Nitroarginine
Conductance
Intravenous Infusions
Nitric Oxide Synthase
Precursor
Inhibitor
Arterial Pressure
Cats
Availability

Keywords

  • Blood pressure
  • Cat
  • Endothelium
  • In vivo
  • L-arginine
  • Myocardial blood flow
  • N-nitro-L-arginine
  • Nitric oxide

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Physiology (medical)
  • Physiology

Cite this

Benyó, Z., Kiss, G., Szabo, C., Csáki, C., & Kovách, A. G. B. (1991). Importance of basal nitric oxide synthesis in regulation of myocardial blood flow. Cardiovascular Research, 25(8), 700-703.

Importance of basal nitric oxide synthesis in regulation of myocardial blood flow. / Benyó, Zoltán; Kiss, Gergely; Szabo, Csaba; Csáki, Csilla; Kovách, Arisztid G B.

In: Cardiovascular Research, Vol. 25, No. 8, 1991, p. 700-703.

Research output: Contribution to journalArticle

Benyó, Z, Kiss, G, Szabo, C, Csáki, C & Kovách, AGB 1991, 'Importance of basal nitric oxide synthesis in regulation of myocardial blood flow', Cardiovascular Research, vol. 25, no. 8, pp. 700-703.
Benyó Z, Kiss G, Szabo C, Csáki C, Kovách AGB. Importance of basal nitric oxide synthesis in regulation of myocardial blood flow. Cardiovascular Research. 1991;25(8):700-703.
Benyó, Zoltán ; Kiss, Gergely ; Szabo, Csaba ; Csáki, Csilla ; Kovách, Arisztid G B. / Importance of basal nitric oxide synthesis in regulation of myocardial blood flow. In: Cardiovascular Research. 1991 ; Vol. 25, No. 8. pp. 700-703.
@article{a7d24c8b6e9f4018879223f114051a55,
title = "Importance of basal nitric oxide synthesis in regulation of myocardial blood flow",
abstract = "Study objective - The aim was to investigate whether basal coronary vascular tone and myocardial perfusion depend upon endothelial nitric oxide (NO) synthesis. Design - Myocardial blood flow and vascular resistance of the left and right ventricles were studied before and after intravenous infusions of either NG-nitro-L-arginine (L-NA), a specific inhibitor of NO synthase, or L-arginine, the precursor of NO synthesis. Radiolabelled microspheres were used to study myocardial blood flow in small tissue sections. Experimental material - 14 anaesthetised male cats, weight 2.1-3.5 kg, were used. Measurements and main results - Measurements were made before and 15 and 40 min after L-NA treatment (30 mg·kg-1 bolus followed by 1 mg·kg-1·min-1 infusion; n=8), and before and 15 min after L-arginine treatment (30 mg·kg-1 bolus followed by 10 mg·kg-1·min-1 infusion; n=6). L-NA significantly reduced coronary blood flow to the left and right ventricle, by 30(SEM 9) and 48(6){\%} respectively, after 15 min, but only to the right ventricle, by 45(8){\%}, after 40 min. Mean arterial pressure and myocardial vascular resistance were raised during the L-NA infusion. In contrast, L-arginine did not elicit any change in the variables studied. Conclusions - The conductance of the coronary vascular bed and the resting myocardial blood flow is regulated by L-arginine derived nitric oxide, and exogenous L-arginine availability is not a limiting factor in this NO generation.",
keywords = "Blood pressure, Cat, Endothelium, In vivo, L-arginine, Myocardial blood flow, N-nitro-L-arginine, Nitric oxide",
author = "Zolt{\'a}n Beny{\'o} and Gergely Kiss and Csaba Szabo and Csilla Cs{\'a}ki and Kov{\'a}ch, {Arisztid G B}",
year = "1991",
language = "English (US)",
volume = "25",
pages = "700--703",
journal = "Cardiovascular Research",
issn = "0008-6363",
publisher = "Oxford University Press",
number = "8",

}

TY - JOUR

T1 - Importance of basal nitric oxide synthesis in regulation of myocardial blood flow

AU - Benyó, Zoltán

AU - Kiss, Gergely

AU - Szabo, Csaba

AU - Csáki, Csilla

AU - Kovách, Arisztid G B

PY - 1991

Y1 - 1991

N2 - Study objective - The aim was to investigate whether basal coronary vascular tone and myocardial perfusion depend upon endothelial nitric oxide (NO) synthesis. Design - Myocardial blood flow and vascular resistance of the left and right ventricles were studied before and after intravenous infusions of either NG-nitro-L-arginine (L-NA), a specific inhibitor of NO synthase, or L-arginine, the precursor of NO synthesis. Radiolabelled microspheres were used to study myocardial blood flow in small tissue sections. Experimental material - 14 anaesthetised male cats, weight 2.1-3.5 kg, were used. Measurements and main results - Measurements were made before and 15 and 40 min after L-NA treatment (30 mg·kg-1 bolus followed by 1 mg·kg-1·min-1 infusion; n=8), and before and 15 min after L-arginine treatment (30 mg·kg-1 bolus followed by 10 mg·kg-1·min-1 infusion; n=6). L-NA significantly reduced coronary blood flow to the left and right ventricle, by 30(SEM 9) and 48(6)% respectively, after 15 min, but only to the right ventricle, by 45(8)%, after 40 min. Mean arterial pressure and myocardial vascular resistance were raised during the L-NA infusion. In contrast, L-arginine did not elicit any change in the variables studied. Conclusions - The conductance of the coronary vascular bed and the resting myocardial blood flow is regulated by L-arginine derived nitric oxide, and exogenous L-arginine availability is not a limiting factor in this NO generation.

AB - Study objective - The aim was to investigate whether basal coronary vascular tone and myocardial perfusion depend upon endothelial nitric oxide (NO) synthesis. Design - Myocardial blood flow and vascular resistance of the left and right ventricles were studied before and after intravenous infusions of either NG-nitro-L-arginine (L-NA), a specific inhibitor of NO synthase, or L-arginine, the precursor of NO synthesis. Radiolabelled microspheres were used to study myocardial blood flow in small tissue sections. Experimental material - 14 anaesthetised male cats, weight 2.1-3.5 kg, were used. Measurements and main results - Measurements were made before and 15 and 40 min after L-NA treatment (30 mg·kg-1 bolus followed by 1 mg·kg-1·min-1 infusion; n=8), and before and 15 min after L-arginine treatment (30 mg·kg-1 bolus followed by 10 mg·kg-1·min-1 infusion; n=6). L-NA significantly reduced coronary blood flow to the left and right ventricle, by 30(SEM 9) and 48(6)% respectively, after 15 min, but only to the right ventricle, by 45(8)%, after 40 min. Mean arterial pressure and myocardial vascular resistance were raised during the L-NA infusion. In contrast, L-arginine did not elicit any change in the variables studied. Conclusions - The conductance of the coronary vascular bed and the resting myocardial blood flow is regulated by L-arginine derived nitric oxide, and exogenous L-arginine availability is not a limiting factor in this NO generation.

KW - Blood pressure

KW - Cat

KW - Endothelium

KW - In vivo

KW - L-arginine

KW - Myocardial blood flow

KW - N-nitro-L-arginine

KW - Nitric oxide

UR - http://www.scopus.com/inward/record.url?scp=0025768215&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025768215&partnerID=8YFLogxK

M3 - Article

C2 - 1913760

VL - 25

SP - 700

EP - 703

JO - Cardiovascular Research

JF - Cardiovascular Research

SN - 0008-6363

IS - 8

ER -