Improved detection of Bartonella DNA in mammalian hosts and arthropod vectors by real-time PCR using the NADH dehydrogenase gamma subunit (nuoG)

James M. Colborn, Michael Y. Kosoy, Vladimir Motin, Maxim V. Telepnev, Gustavo Valbuena, Khin S. Myint, Yuriy Fofanov, Catherine Putonti, Chen Feng, Leonard Peruski

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

We used a whole-genome scanning technique to identify the NADH dehydrogenase gamma subunit (nuoG) primer set that is sensitive and specific enough to detect a diverse number of Bartonella species in a wide range of environmental samples yet maintains minimal cross-reactivity to mammalian host and arthropod vector organisms.

Original languageEnglish (US)
Pages (from-to)4630-4633
Number of pages4
JournalJournal of Clinical Microbiology
Volume48
Issue number12
DOIs
StatePublished - Dec 2010

Fingerprint

Arthropod Vectors
Bartonella
NADH Dehydrogenase
Real-Time Polymerase Chain Reaction
Genome
DNA

ASJC Scopus subject areas

  • Microbiology (medical)

Cite this

Improved detection of Bartonella DNA in mammalian hosts and arthropod vectors by real-time PCR using the NADH dehydrogenase gamma subunit (nuoG). / Colborn, James M.; Kosoy, Michael Y.; Motin, Vladimir; Telepnev, Maxim V.; Valbuena, Gustavo; Myint, Khin S.; Fofanov, Yuriy; Putonti, Catherine; Feng, Chen; Peruski, Leonard.

In: Journal of Clinical Microbiology, Vol. 48, No. 12, 12.2010, p. 4630-4633.

Research output: Contribution to journalArticle

Colborn, James M. ; Kosoy, Michael Y. ; Motin, Vladimir ; Telepnev, Maxim V. ; Valbuena, Gustavo ; Myint, Khin S. ; Fofanov, Yuriy ; Putonti, Catherine ; Feng, Chen ; Peruski, Leonard. / Improved detection of Bartonella DNA in mammalian hosts and arthropod vectors by real-time PCR using the NADH dehydrogenase gamma subunit (nuoG). In: Journal of Clinical Microbiology. 2010 ; Vol. 48, No. 12. pp. 4630-4633.
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