Improved detection of Bartonella DNA in mammalian hosts and arthropod vectors by real-time PCR using the NADH dehydrogenase gamma subunit (nuoG)

James M. Colborn, Michael Y. Kosoy, Vladimir L. Motin, Maxim V. Telepnev, Gustavo A Valbuena, Khin S. Myint, Yuri Fofanov, Catherine Putonti, Chen Feng, Leonard Peruski

Research output: Contribution to journalArticle

29 Scopus citations

Abstract

We used a whole-genome scanning technique to identify the NADH dehydrogenase gamma subunit (nuoG) primer set that is sensitive and specific enough to detect a diverse number of Bartonella species in a wide range of environmental samples yet maintains minimal cross-reactivity to mammalian host and arthropod vector organisms.

Original languageEnglish (US)
Pages (from-to)4630-4633
Number of pages4
JournalJournal of Clinical Microbiology
Volume48
Issue number12
DOIs
StatePublished - Dec 1 2010

ASJC Scopus subject areas

  • Microbiology (medical)

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    Colborn, J. M., Kosoy, M. Y., Motin, V. L., Telepnev, M. V., Valbuena, G. A., Myint, K. S., Fofanov, Y., Putonti, C., Feng, C., & Peruski, L. (2010). Improved detection of Bartonella DNA in mammalian hosts and arthropod vectors by real-time PCR using the NADH dehydrogenase gamma subunit (nuoG). Journal of Clinical Microbiology, 48(12), 4630-4633. https://doi.org/10.1128/JCM.00470-10