Improved detection of Bartonella DNA in mammalian hosts and arthropod vectors by real-time PCR using the NADH dehydrogenase gamma subunit (nuoG)

James M. Colborn; Michael Y. Kosoy; Vladimir L. Motin; Maxim V. Telepnev; Gustavo Valbuena; Khin S. Myint; Yuri Fofanov; Catherine Putonti; Chen Feng; Leonard Peruski

doi:10.1128/JCM.00470-10

Improved detection of Bartonella DNA in mammalian hosts and arthropod vectors by real-time PCR using the NADH dehydrogenase gamma subunit (nuoG)

James M. Colborn
, Michael Y. Kosoy
, Vladimir L. Motin
, Maxim V. Telepnev
, Gustavo Valbuena
, Khin S. Myint
, Yuri Fofanov
, Catherine Putonti
, Chen Feng
, Leonard Peruski

Pathology

Research output: Contribution to journal › Article › peer-review

72 Scopus citations

Abstract

We used a whole-genome scanning technique to identify the NADH dehydrogenase gamma subunit (nuoG) primer set that is sensitive and specific enough to detect a diverse number of Bartonella species in a wide range of environmental samples yet maintains minimal cross-reactivity to mammalian host and arthropod vector organisms.

Original language	English (US)
Pages (from-to)	4630-4633
Number of pages	4
Journal	Journal of Clinical Microbiology
Volume	48
Issue number	12
DOIs	https://doi.org/10.1128/JCM.00470-10
State	Published - Dec 2010

ASJC Scopus subject areas

Microbiology (medical)

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10.1128/JCM.00470-10

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Colborn, J. M., Kosoy, M. Y., Motin, V. L., Telepnev, M. V., Valbuena, G., Myint, K. S., Fofanov, Y., Putonti, C., Feng, C., & Peruski, L. (2010). Improved detection of Bartonella DNA in mammalian hosts and arthropod vectors by real-time PCR using the NADH dehydrogenase gamma subunit (nuoG). Journal of Clinical Microbiology, 48(12), 4630-4633. https://doi.org/10.1128/JCM.00470-10

Colborn, JM, Kosoy, MY, Motin, VL, Telepnev, MV, Valbuena, G, Myint, KS, Fofanov, Y, Putonti, C, Feng, C & Peruski, L 2010, 'Improved detection of Bartonella DNA in mammalian hosts and arthropod vectors by real-time PCR using the NADH dehydrogenase gamma subunit (nuoG)', Journal of Clinical Microbiology, vol. 48, no. 12, pp. 4630-4633. https://doi.org/10.1128/JCM.00470-10

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AU - Motin, Vladimir L.

AU - Telepnev, Maxim V.

AU - Valbuena, Gustavo

AU - Myint, Khin S.

AU - Fofanov, Yuri

AU - Putonti, Catherine

AU - Feng, Chen

AU - Peruski, Leonard

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Improved detection of Bartonella DNA in mammalian hosts and arthropod vectors by real-time PCR using the NADH dehydrogenase gamma subunit (nuoG)

Abstract

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