Improved methods to detect low levels of HIV using antibody-based technologies

Eliseo Eugenin, Joan W. Berman

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Citation (Scopus)

Abstract

Persistence of latent virus represents a major barrier to eradicating HIV even in the current antiretroviral therapy era. A critical limitation to eliminating these viral reservoirs is the lack of reliable methods to detect, quantify, and characterize cells harboring low levels of virus. However, recent work of several laboratories indicates that PCR and viral amplification based technologies underestimate or overestimate the size of the reservoirs. Thus, new technologies and methodologies to detect, quantify, and characterize these viral reservoirs are necessary to monitor and eradicate HIV. Recent developments in imaging technologies have enabled the development or improvement of detection protocols and have facilitated the identification and quantification of several markers with exquisite resolution. In the context of HIV, we developed new protocols for the detection of low amounts of viral proteins. In this chapter, we describe several antibody-based technologies for signal amplification to improve and detect low amounts of HIV proteins in cells, tissues, and other biological samples. The improvement in these techniques is essential to detect viral reservoirs and to design strategies to eliminate them.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages265-279
Number of pages15
DOIs
StatePublished - Jan 1 2016
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume1354
ISSN (Print)1064-3745

Fingerprint

HIV Antibodies
Technology
HIV
Viruses
Human Immunodeficiency Virus Proteins
Viral Proteins
Polymerase Chain Reaction
Antibodies
Therapeutics

Keywords

  • AIDS
  • Detection
  • Eradication
  • HIV
  • QVOA
  • Reservoirs

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Eugenin, E., & Berman, J. W. (2016). Improved methods to detect low levels of HIV using antibody-based technologies. In Methods in Molecular Biology (pp. 265-279). (Methods in Molecular Biology; Vol. 1354). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-3046-3_18

Improved methods to detect low levels of HIV using antibody-based technologies. / Eugenin, Eliseo; Berman, Joan W.

Methods in Molecular Biology. Humana Press Inc., 2016. p. 265-279 (Methods in Molecular Biology; Vol. 1354).

Research output: Chapter in Book/Report/Conference proceedingChapter

Eugenin, E & Berman, JW 2016, Improved methods to detect low levels of HIV using antibody-based technologies. in Methods in Molecular Biology. Methods in Molecular Biology, vol. 1354, Humana Press Inc., pp. 265-279. https://doi.org/10.1007/978-1-4939-3046-3_18
Eugenin E, Berman JW. Improved methods to detect low levels of HIV using antibody-based technologies. In Methods in Molecular Biology. Humana Press Inc. 2016. p. 265-279. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-3046-3_18
Eugenin, Eliseo ; Berman, Joan W. / Improved methods to detect low levels of HIV using antibody-based technologies. Methods in Molecular Biology. Humana Press Inc., 2016. pp. 265-279 (Methods in Molecular Biology).
@inbook{a2766957727f45c2b51757916c89e84c,
title = "Improved methods to detect low levels of HIV using antibody-based technologies",
abstract = "Persistence of latent virus represents a major barrier to eradicating HIV even in the current antiretroviral therapy era. A critical limitation to eliminating these viral reservoirs is the lack of reliable methods to detect, quantify, and characterize cells harboring low levels of virus. However, recent work of several laboratories indicates that PCR and viral amplification based technologies underestimate or overestimate the size of the reservoirs. Thus, new technologies and methodologies to detect, quantify, and characterize these viral reservoirs are necessary to monitor and eradicate HIV. Recent developments in imaging technologies have enabled the development or improvement of detection protocols and have facilitated the identification and quantification of several markers with exquisite resolution. In the context of HIV, we developed new protocols for the detection of low amounts of viral proteins. In this chapter, we describe several antibody-based technologies for signal amplification to improve and detect low amounts of HIV proteins in cells, tissues, and other biological samples. The improvement in these techniques is essential to detect viral reservoirs and to design strategies to eliminate them.",
keywords = "AIDS, Detection, Eradication, HIV, QVOA, Reservoirs",
author = "Eliseo Eugenin and Berman, {Joan W.}",
year = "2016",
month = "1",
day = "1",
doi = "10.1007/978-1-4939-3046-3_18",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "265--279",
booktitle = "Methods in Molecular Biology",

}

TY - CHAP

T1 - Improved methods to detect low levels of HIV using antibody-based technologies

AU - Eugenin, Eliseo

AU - Berman, Joan W.

PY - 2016/1/1

Y1 - 2016/1/1

N2 - Persistence of latent virus represents a major barrier to eradicating HIV even in the current antiretroviral therapy era. A critical limitation to eliminating these viral reservoirs is the lack of reliable methods to detect, quantify, and characterize cells harboring low levels of virus. However, recent work of several laboratories indicates that PCR and viral amplification based technologies underestimate or overestimate the size of the reservoirs. Thus, new technologies and methodologies to detect, quantify, and characterize these viral reservoirs are necessary to monitor and eradicate HIV. Recent developments in imaging technologies have enabled the development or improvement of detection protocols and have facilitated the identification and quantification of several markers with exquisite resolution. In the context of HIV, we developed new protocols for the detection of low amounts of viral proteins. In this chapter, we describe several antibody-based technologies for signal amplification to improve and detect low amounts of HIV proteins in cells, tissues, and other biological samples. The improvement in these techniques is essential to detect viral reservoirs and to design strategies to eliminate them.

AB - Persistence of latent virus represents a major barrier to eradicating HIV even in the current antiretroviral therapy era. A critical limitation to eliminating these viral reservoirs is the lack of reliable methods to detect, quantify, and characterize cells harboring low levels of virus. However, recent work of several laboratories indicates that PCR and viral amplification based technologies underestimate or overestimate the size of the reservoirs. Thus, new technologies and methodologies to detect, quantify, and characterize these viral reservoirs are necessary to monitor and eradicate HIV. Recent developments in imaging technologies have enabled the development or improvement of detection protocols and have facilitated the identification and quantification of several markers with exquisite resolution. In the context of HIV, we developed new protocols for the detection of low amounts of viral proteins. In this chapter, we describe several antibody-based technologies for signal amplification to improve and detect low amounts of HIV proteins in cells, tissues, and other biological samples. The improvement in these techniques is essential to detect viral reservoirs and to design strategies to eliminate them.

KW - AIDS

KW - Detection

KW - Eradication

KW - HIV

KW - QVOA

KW - Reservoirs

UR - http://www.scopus.com/inward/record.url?scp=84952912282&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84952912282&partnerID=8YFLogxK

U2 - 10.1007/978-1-4939-3046-3_18

DO - 10.1007/978-1-4939-3046-3_18

M3 - Chapter

C2 - 26714718

AN - SCOPUS:84952912282

T3 - Methods in Molecular Biology

SP - 265

EP - 279

BT - Methods in Molecular Biology

PB - Humana Press Inc.

ER -