Improved NMR spectra of a protein-DNA complex through rational mutagenesis and the application of a sensitivity optimized isotope-filtered NOESY experiment

Keyphrases

• Protein-DNA Complex 100%
• NOE Experiments 100%
• NMR Spectra 100%
• Structure-guided mutagenesis 100%
• Sensitivity-optimized 100%
• Line Broadening 66%
• DNA-binding Domain 33%
• Aromatic Ring 33%
• Dynamic Change 33%
• Protein Complex 33%
• Biochemical Approaches 33%
• Chemical Shift 33%
• DNA Complex 33%
• Ringer 33%
• DNA Binding Sites 33%
• Aromatic Residues 33%
• Spectral Changes 33%
• New Sequences 33%
• Spectroscopic Means 33%
• Inversion Pulse 33%
• Targeted Replacement 33%
• Spectrum Optimization 33%

Biochemistry, Genetics and Molecular Biology

• DNA Protein Complex 100%
• Mutagenesis 100%
• Nuclear Overhauser Effect Spectroscopy 100%
• Pulse Rate 66%
• DNA-binding Domain 33%
• Protein Complexes 33%
• DNA Binding Site 33%
• Adiabaticity 33%

Chemistry

• Nuclear Overhauser Enhanced Spectroscopy 100%
• NMR Spectrum 100%
• DNA 100%
• Line Broadening 40%
• DNA Binding 40%
• Binding Site 20%
• NMR Spectroscopy 20%
• Chemical Shift 20%
• Aromatic Structure 20%
• Protein Complex 20%