Improved reverse transcription and amplification of flaviviral RNA following low level methyl mercury hydroxide denaturation

J. E. Whitby, A. D. Jennings, Alan Barrett

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The reverse transcription and amplification of the RNA of viruses of the Flaviviridae have been notably enhanced by the addition of a low level methyl mercury hydroxide denaturation of the viral RNA. Calculated levels of methyl mercury hydroxide, markedly lower than those normally recommended, were used. The methodology utilised vRNA from virions crudely purified from tissue culture supernatant by centrifugation only. The addition of this denaturation protocol has enabled the routine amplification of regions up to 1.6 kilobases from the RNA of a number of flaviviruses.

Original languageEnglish (US)
Pages (from-to)193-197
Number of pages5
JournalMethods in Molecular and Cellular Biology
Volume3
Issue number4
StatePublished - 1992
Externally publishedYes

Fingerprint

Denaturation
Transcription
Mercury
Reverse Transcription
Amplification
Flaviviridae
RNA
Flavivirus
Tissue culture
Centrifugation
RNA Viruses
Viral RNA
Viruses
Virion
hydroxide ion

ASJC Scopus subject areas

  • Cell Biology
  • Clinical Biochemistry
  • Molecular Biology

Cite this

Improved reverse transcription and amplification of flaviviral RNA following low level methyl mercury hydroxide denaturation. / Whitby, J. E.; Jennings, A. D.; Barrett, Alan.

In: Methods in Molecular and Cellular Biology, Vol. 3, No. 4, 1992, p. 193-197.

Research output: Contribution to journalArticle

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