Improved reverse transcription and amplification of flaviviral RNA following low level methyl mercury hydroxide denaturation

J. E. Whitby, A. D. Jennings, A. D.T. Barrett

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

The reverse transcription and amplification of the RNA of viruses of the Flaviviridae have been notably enhanced by the addition of a low level methyl mercury hydroxide denaturation of the viral RNA. Calculated levels of methyl mercury hydroxide, markedly lower than those normally recommended, were used. The methodology utilised vRNA from virions crudely purified from tissue culture supernatant by centrifugation only. The addition of this denaturation protocol has enabled the routine amplification of regions up to 1.6 kilobases from the RNA of a number of flaviviruses.

Original languageEnglish (US)
Pages (from-to)193-197
Number of pages5
JournalMethods in Molecular and Cellular Biology
Volume3
Issue number4
StatePublished - Dec 1 1992
Externally publishedYes

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ASJC Scopus subject areas

  • Molecular Biology
  • Clinical Biochemistry
  • Cell Biology

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