Abstract
The reverse transcription and amplification of the RNA of viruses of the Flaviviridae have been notably enhanced by the addition of a low level methyl mercury hydroxide denaturation of the viral RNA. Calculated levels of methyl mercury hydroxide, markedly lower than those normally recommended, were used. The methodology utilised vRNA from virions crudely purified from tissue culture supernatant by centrifugation only. The addition of this denaturation protocol has enabled the routine amplification of regions up to 1.6 kilobases from the RNA of a number of flaviviruses.
Original language | English (US) |
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Pages (from-to) | 193-197 |
Number of pages | 5 |
Journal | Methods in Molecular and Cellular Biology |
Volume | 3 |
Issue number | 4 |
State | Published - 1992 |
Externally published | Yes |
ASJC Scopus subject areas
- Molecular Biology
- Clinical Biochemistry
- Cell Biology