Abstract
In glioma surgery, Protoporphyrin IX (PpIX) fluorescence may identify residual tumor that could be resected while minimizing damage to normal brain. We demonstrate that improved sensitivity for wide-field spectroscopic fluorescence imaging is achieved with minimal disruption to the neurosurgical workflow using an electron-multiplying charge-coupled device (EMCCD) relative to a state-of-the-art CMOS system. In phantom experiments the EMCCD system can detect at least two orders-ofmagnitude lower PpIX. Ex vivo tissue imaging on a rat glioma model demonstrates improved fluorescence contrast compared with neurosurgical fluorescence microscope technology, and the fluorescence detection is confirmed with measurements from a clinically-validated spectroscopic probe. Greater PpIX sensitivity in wide-field fluorescence imaging may improve the residual tumor detection during surgery with consequent impact on survival.
Original language | English (US) |
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Article number | 252150 |
Pages (from-to) | 5063-5074 |
Number of pages | 12 |
Journal | Biomedical Optics Express |
Volume | 6 |
Issue number | 12 |
DOIs | |
State | Published - Nov 30 2015 |
Externally published | Yes |
Keywords
- Medical optics instrumentation
- Spectroscopy, fluorescence and luminescence
ASJC Scopus subject areas
- Biotechnology
- Atomic and Molecular Physics, and Optics