Improved separation of lipid esters by thin-layer chromatography

Hari K. Bhat, Ghulam Ansari

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Methods for the separation of ethyl- and 2-chloroethyl esters of fatty acids using argentation thin-layer chromatography (TLC) and linear and circular reversed-phase TLC are described. An argentation TLC method for the separatio nof cholesterol esters is also described. The separation is based on the degree of unsaturation in the fatty acid moiety. In all instances good and reproducible resolution was achieved by unidirectional single developments at room temperature with reduced analysis times (9 min for argentation TLC, 7 min for circular reversed-phase TLC).

Original languageEnglish (US)
Pages (from-to)369-378
Number of pages10
JournalJournal of Chromatography A
Volume483
Issue numberC
DOIs
StatePublished - 1989

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Thin layer chromatography
Thin Layer Chromatography
Esters
Lipids
Reverse-Phase Chromatography
Fatty Acids
Cholesterol Esters
Temperature

ASJC Scopus subject areas

  • Analytical Chemistry
  • Clinical Biochemistry
  • Molecular Medicine

Cite this

Improved separation of lipid esters by thin-layer chromatography. / Bhat, Hari K.; Ansari, Ghulam.

In: Journal of Chromatography A, Vol. 483, No. C, 1989, p. 369-378.

Research output: Contribution to journalArticle

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