Improved separation of lipid esters by thin-layer chromatography

Hari K. Bhat, G. A.S. Ansari

Research output: Contribution to journalArticlepeer-review

18 Scopus citations


Methods for the separation of ethyl- and 2-chloroethyl esters of fatty acids using argentation thin-layer chromatography (TLC) and linear and circular reversed-phase TLC are described. An argentation TLC method for the separatio nof cholesterol esters is also described. The separation is based on the degree of unsaturation in the fatty acid moiety. In all instances good and reproducible resolution was achieved by unidirectional single developments at room temperature with reduced analysis times (9 min for argentation TLC, 7 min for circular reversed-phase TLC).

Original languageEnglish (US)
Pages (from-to)369-378
Number of pages10
JournalJournal of Chromatography A
Issue numberC
StatePublished - 1989
Externally publishedYes

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry


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