TY - CHAP
T1 - In Silico Prediction of MYO1C-Rhodopsin Interactions and Its Significance in Protein Localization and Visual Function
AU - Lobo, Glenn P.
AU - Radhakrishnan, Rakesh
AU - Leung, Matthias
AU - Gruesen, Andrew
AU - Knölker, Hans Joachim
AU - van Kuijk, Frederik J.
AU - Montezuma, Sandra R.
N1 - Publisher Copyright:
© 2023, The Author(s), under exclusive license to Springer Nature Switzerland AG.
PY - 2023
Y1 - 2023
N2 - Rods and cones are photoreceptor neurons in the retina that are required for visual sensation in vertebrates, where proper protein localization and compartmentalization are critical for phototransduction and visual function. In human retinal diseases, improper protein transport to the outer segment (OS) or mislocalization of proteins to the inner segment (IS) could lead to impaired visual responses and photoreceptor cell degeneration, causing a loss of visual function. We showed involvement of an unconventional motor protein, MYO1C, in the proper localization of rhodopsin to the OS, where loss of MYO1C in a mammalian model caused mislocalization of rhodopsin to IS and cell bodies, leading to progressively severe retinal phenotypes. In this study, using modeling and docking analysis, we aimed to identify the protein–protein interaction sites between MYO1C and Rhodopsin to establish a hypothesis that a physical interaction between these proteins is necessary for the proper trafficking of rhodopsin and visual function.
AB - Rods and cones are photoreceptor neurons in the retina that are required for visual sensation in vertebrates, where proper protein localization and compartmentalization are critical for phototransduction and visual function. In human retinal diseases, improper protein transport to the outer segment (OS) or mislocalization of proteins to the inner segment (IS) could lead to impaired visual responses and photoreceptor cell degeneration, causing a loss of visual function. We showed involvement of an unconventional motor protein, MYO1C, in the proper localization of rhodopsin to the OS, where loss of MYO1C in a mammalian model caused mislocalization of rhodopsin to IS and cell bodies, leading to progressively severe retinal phenotypes. In this study, using modeling and docking analysis, we aimed to identify the protein–protein interaction sites between MYO1C and Rhodopsin to establish a hypothesis that a physical interaction between these proteins is necessary for the proper trafficking of rhodopsin and visual function.
KW - Motor protein
KW - Myosin 1C
KW - Outer segments
KW - Photoreceptor
KW - Protein localization
KW - Retina
KW - Rhodopsin
KW - Visual function
UR - https://www.scopus.com/pages/publications/85164847178
UR - https://www.scopus.com/pages/publications/85164847178#tab=citedBy
U2 - 10.1007/978-3-031-27681-1_73
DO - 10.1007/978-3-031-27681-1_73
M3 - Chapter
C2 - 37440078
AN - SCOPUS:85164847178
T3 - Advances in Experimental Medicine and Biology
SP - 499
EP - 505
BT - Advances in Experimental Medicine and Biology
PB - Springer
ER -