In situ cytokines (IL-4, IL-10, IL-12, IFN-γ) and chemokines (MCP-1, MIP-1α) gene expression in human Leishmania (Leishmania) mexicana infection

Guillermo Valencia-Pacheco, Elsy Nalleli Loría-Cervera, Erika Ivett Sosa-Bibiano, Elsy B. Canché-Pool, Alberto Vargas-Gonzalez, Peter Melby, Fernando J. Andrade-Narvaez

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Crucial to the defense against Leishmania is the ability of the host to mount a cell-mediated immune response capable of controlling and/or eliminating the parasite. The composition of the cell populations recruited in the early phase of the infection seems to be essential for defining the infection outcomes. The signals that initiate and regulate the early immune response and local accumulation of cell subsets in the skin are poorly understood. We previously studied the in situ expression of cytokine genes in patients with localized cutaneous leishmaniasis (LCL) caused by Leishmania ( Leishmania) mexicana. In the present study we examined in situ cytokine (IL-4, IL-10, IL-12, IFN-γ) and chemokine (MCP-1, MIP-1α) gene expression in L. ( L.) mexicana active LCL lesions, and in the delayed type hypersensitivity (DTH) skin response to Leishmania antigen in subjects with healed lesion and subclinical infection. Data regarding cytokines were similar to previous studies in patients with active LCL. There were no significant differences in the profile of cytokine and chemokine gene expression in DTH from subjects with healed or subclinical infection. IL-12 gene expression detected in both groups was similar. High expression of MCP-1 was detected in all patients with active LCL. There was no difference in the level of MCP-1 expression between the healed lesion and the subclinical infection groups ( p= 0.876). IL-12 and MCP-1 in the absence of IFN-γ might be playing a crucial role in infection outcomes at skin level.

Original languageEnglish (US)
Pages (from-to)56-61
Number of pages6
JournalCytokine
Volume69
Issue number1
DOIs
StatePublished - 2014

Fingerprint

Leishmania mexicana
Cutaneous Leishmaniasis
Interleukin-12
Chemokines
Gene expression
Interleukin-4
Interleukin-10
Asymptomatic Infections
Cytokines
Gene Expression
Skin
Leishmania
Delayed Hypersensitivity
Infection
Cells
Parasites
Genes
Antigens
Chemical analysis
Population

Keywords

  • Chemokines
  • Cytokines
  • In situ
  • Leishmania mexicana
  • Localized cutaneous leishmaniasis

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy
  • Hematology
  • Biochemistry
  • Molecular Biology

Cite this

Valencia-Pacheco, G., Loría-Cervera, E. N., Sosa-Bibiano, E. I., Canché-Pool, E. B., Vargas-Gonzalez, A., Melby, P., & Andrade-Narvaez, F. J. (2014). In situ cytokines (IL-4, IL-10, IL-12, IFN-γ) and chemokines (MCP-1, MIP-1α) gene expression in human Leishmania (Leishmania) mexicana infection. Cytokine, 69(1), 56-61. https://doi.org/10.1016/j.cyto.2014.05.016

In situ cytokines (IL-4, IL-10, IL-12, IFN-γ) and chemokines (MCP-1, MIP-1α) gene expression in human Leishmania (Leishmania) mexicana infection. / Valencia-Pacheco, Guillermo; Loría-Cervera, Elsy Nalleli; Sosa-Bibiano, Erika Ivett; Canché-Pool, Elsy B.; Vargas-Gonzalez, Alberto; Melby, Peter; Andrade-Narvaez, Fernando J.

In: Cytokine, Vol. 69, No. 1, 2014, p. 56-61.

Research output: Contribution to journalArticle

Valencia-Pacheco, G, Loría-Cervera, EN, Sosa-Bibiano, EI, Canché-Pool, EB, Vargas-Gonzalez, A, Melby, P & Andrade-Narvaez, FJ 2014, 'In situ cytokines (IL-4, IL-10, IL-12, IFN-γ) and chemokines (MCP-1, MIP-1α) gene expression in human Leishmania (Leishmania) mexicana infection', Cytokine, vol. 69, no. 1, pp. 56-61. https://doi.org/10.1016/j.cyto.2014.05.016
Valencia-Pacheco, Guillermo ; Loría-Cervera, Elsy Nalleli ; Sosa-Bibiano, Erika Ivett ; Canché-Pool, Elsy B. ; Vargas-Gonzalez, Alberto ; Melby, Peter ; Andrade-Narvaez, Fernando J. / In situ cytokines (IL-4, IL-10, IL-12, IFN-γ) and chemokines (MCP-1, MIP-1α) gene expression in human Leishmania (Leishmania) mexicana infection. In: Cytokine. 2014 ; Vol. 69, No. 1. pp. 56-61.
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AU - Loría-Cervera, Elsy Nalleli

AU - Sosa-Bibiano, Erika Ivett

AU - Canché-Pool, Elsy B.

AU - Vargas-Gonzalez, Alberto

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AU - Andrade-Narvaez, Fernando J.

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