In situ expression of interleukin-10 and interleukin-12 in active human cutaneous leishmaniasis

Peter C. Melby, Fernando Andrade-Narvaez, Barbara J. Darnell, Guillermo Valencia-Pacheco

Research output: Contribution to journalArticle

37 Scopus citations

Abstract

Th1-type cellular immune responses (interferon-γ) play a critical role in protection against Leishmania spp. infection, whereas Th2-type cytokines (interleukin (IL)-4, IL-10) have a counter-protective effect. IL-12, a potent inducer of Th1-type cellular immune responses, may play a pivotal role in the development of a protective response. We found that IL-10 and IL-12 mRNAs were expressed in most lesions of individuals with active cutaneous leishmaniasis. The quantity of IL-12 mRNA was highly variable but correlated strongly with the level of interferon-γ expression. IL-12 expression also paralleled the expression of IL-10, a potent in vitro suppressor of IL-12 and interferon-γ production. The more chronic, non-healing lesions generally had higher levels of IL-12 mRNA indicating that the expression of this cytokine alone was not sufficient to induce healing. Although the in situ production of IL-10 did not appear to block IL-12 expression, IL-10 may still promote disease by direct suppression of macrophage activation.

Original languageEnglish (US)
Pages (from-to)101-107
Number of pages7
JournalFEMS Immunology and Medical Microbiology
Volume15
Issue number2-3
DOIs
StatePublished - Sep 1996
Externally publishedYes

Keywords

  • Cellular immunity
  • Cytokine
  • Interleukin-10
  • Interleukin-12
  • Leishmania
  • Polymerase chain reaction

ASJC Scopus subject areas

  • Immunology and Allergy
  • Microbiology
  • Immunology
  • Microbiology (medical)
  • Infectious Diseases

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