In situ hybridization for the detection of hepatitis C virus RNA in human liver tissue

G. Li, K. Li, Alfred Lea, N. L. Li, N. E. Abdulla, M. A. Eltorky, Monique Ferguson

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

In situ hybridization (ISH) enables visualization of specific nucleic acid in morphologically preserved cells and tissue sections. Detection of the HCV genomes in clinical specimens is useful for differential diagnosis, particularly between recurrent HCV infection and acute cellular rejection in transplant specimens. We optimized an ISH protocol that demonstrated sensitivity and specificity for detecting genomic and replicative form of HCV RNA in tissue biopsies. Digoxigenin (Dig)-labelled sense and anti-sense riboprobes were synthesized using a plasmid containing a fragment of the highly conserved HCV noncoding region as a template. The efficiency of the Dig-labelled riboprobes in detecting genomic and replicative-intermediate HCV RNA was analysed in 30 liver biopsies from patients infected or uninfected with HCV in a blinded study. A Huh7 cell line that stably replicates genome-length HCV RNA was developed to be used as a positive control. Negative control riboprobes were used in parallel to evaluate and control for background staining. The anti-sense probe detected HCV RNA in 20/21 specimens from HCV-infected liver tissues obtained from patients and in 0/9 samples from patients with non-HCV-related liver diseases, resulting in a sensitivity and specificity of 95% and 100%, respectively. HCV genomic RNA was variably distributed in tissue sections and was located primarily in the perinuclear regions in hepatocytes. Detection of HCV RNA by our optimized ISH protocol appears to be a sensitive and specific method when processing clinical specimens. It may also be revealing when exploring the pathophysiology of HCV infection by verifying the presence of viral genetic material within heptocytes and other cellular elements of diseased liver tissue. This methodology might also evaluate the response to antiviral therapies by demonstrating the absence or alteration of genetic material in clinical specimens from successfully treated patients.

Original languageEnglish (US)
Pages (from-to)183-192
Number of pages10
JournalJournal of Viral Hepatitis
Volume20
Issue number3
DOIs
StatePublished - Mar 2013

Fingerprint

Hepacivirus
In Situ Hybridization
RNA
Liver
Digoxigenin
Liver Diseases
Genome
Biopsy
Sensitivity and Specificity
Graft Rejection
Infection
Nucleic Acids
Genes
Antiviral Agents
Hepatocytes
Plasmids
Differential Diagnosis
Staining and Labeling
Cell Line

Keywords

  • digoxigenin-labelled riboprobes
  • HCV replicon
  • hepatitis C virus
  • in situ hybridization

ASJC Scopus subject areas

  • Hepatology
  • Infectious Diseases
  • Virology

Cite this

In situ hybridization for the detection of hepatitis C virus RNA in human liver tissue. / Li, G.; Li, K.; Lea, Alfred; Li, N. L.; Abdulla, N. E.; Eltorky, M. A.; Ferguson, Monique.

In: Journal of Viral Hepatitis, Vol. 20, No. 3, 03.2013, p. 183-192.

Research output: Contribution to journalArticle

Li, G. ; Li, K. ; Lea, Alfred ; Li, N. L. ; Abdulla, N. E. ; Eltorky, M. A. ; Ferguson, Monique. / In situ hybridization for the detection of hepatitis C virus RNA in human liver tissue. In: Journal of Viral Hepatitis. 2013 ; Vol. 20, No. 3. pp. 183-192.
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