In situ super-resolution imaging of genomic DNA with oligoSTORM and oligoDNA-PAINT

Brian J. Beliveau, Alistair N. Boettiger, Guy Nir, Bogdan Bintu, Peng Yin, Xiaowei Zhuang, C. ting Wu

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

OligoSTORM and OligoDNA-PAINT meld the Oligopaint technology for fluorescent in situ hybridization (FISH) with, respectively, Stochastic Optical Reconstruction Microscopy (STORM) and DNA-based Point Accumulation for Imaging in Nanoscale Topography (DNA-PAINT) to enable in situ single-molecule super-resolution imaging of nucleic acids. Both strategies enable ≤20 nm resolution and are appropriate for imaging nanoscale features of the genomes of a wide range of species, including human, mouse, and fruit fly (Drosophila).

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages231-252
Number of pages22
DOIs
StatePublished - 2017
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume1663
ISSN (Print)1064-3745

Keywords

  • Chromatin
  • Chromosomes
  • DNA-PAINT
  • FISH
  • Genome
  • OligoDNA-PAINT
  • Oligopaint
  • OligoSTORM
  • Single-molecule
  • STORM
  • Super-resolution

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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