In vitro analog of human bone marrow from 3D scaffolds with biomimetic inverted colloidal crystal geometry

Joan Nichols, Joaquin Cortiella, Jungwoo Lee, Jean A. Niles, Meghan Cuddihy, Shaopeng Wang, Joseph Bielitzki, Andrea Cantu, Ron Mlcak, Esther Valdivia, Ryan Yancy, Matthew L. McClure, Nicholas A. Kotov

Research output: Contribution to journalArticle

79 Citations (Scopus)

Abstract

In vitro replicas of bone marrow can potentially provide a continuous source of blood cells for transplantation and serve as a laboratory model to examine human immune system dysfunctions and drug toxicology. Here we report the development of an in vitro artificial bone marrow based on a 3D scaffold with inverted colloidal crystal (ICC) geometry mimicking the structural topology of actual bone marrow matrix. To facilitate adhesion of cells, scaffolds were coated with a layer of transparent nanocomposite. After seeding with hematopoietic stem cells (HSCs), ICC scaffolds were capable of supporting expansion of CD34+ HSCs with B-lymphocyte differentiation. Three-dimensional organization was shown to be critical for production of B cells and antigen-specific antibodies. Functionality of bone marrow constructs was confirmed by implantation of matrices containing human CD34+ cells onto the backs of severe combined immunodeficiency (SCID) mice with subsequent generation of human immune cells.

Original languageEnglish (US)
Pages (from-to)1071-1079
Number of pages9
JournalBiomaterials
Volume30
Issue number6
DOIs
StatePublished - Feb 2009

Fingerprint

Biomimetics
Scaffolds (biology)
Bone
Bone Marrow
Scaffolds
Crystals
Geometry
Cells
Hematopoietic Stem Cells
Stem cells
B-Lymphocytes
Nanocomposites
Severe Combined Immunodeficiency
Bone Matrix
Lymphocytes
Immune system
Cell Transplantation
Antigens
Antibodies
Cell Adhesion

Keywords

  • Bone marrow
  • Colloidal crystals
  • Scaffolds
  • Stem cell
  • Tissue engineering

ASJC Scopus subject areas

  • Biomaterials
  • Bioengineering
  • Ceramics and Composites
  • Mechanics of Materials
  • Biophysics

Cite this

In vitro analog of human bone marrow from 3D scaffolds with biomimetic inverted colloidal crystal geometry. / Nichols, Joan; Cortiella, Joaquin; Lee, Jungwoo; Niles, Jean A.; Cuddihy, Meghan; Wang, Shaopeng; Bielitzki, Joseph; Cantu, Andrea; Mlcak, Ron; Valdivia, Esther; Yancy, Ryan; McClure, Matthew L.; Kotov, Nicholas A.

In: Biomaterials, Vol. 30, No. 6, 02.2009, p. 1071-1079.

Research output: Contribution to journalArticle

Nichols, J, Cortiella, J, Lee, J, Niles, JA, Cuddihy, M, Wang, S, Bielitzki, J, Cantu, A, Mlcak, R, Valdivia, E, Yancy, R, McClure, ML & Kotov, NA 2009, 'In vitro analog of human bone marrow from 3D scaffolds with biomimetic inverted colloidal crystal geometry', Biomaterials, vol. 30, no. 6, pp. 1071-1079. https://doi.org/10.1016/j.biomaterials.2008.10.041
Nichols, Joan ; Cortiella, Joaquin ; Lee, Jungwoo ; Niles, Jean A. ; Cuddihy, Meghan ; Wang, Shaopeng ; Bielitzki, Joseph ; Cantu, Andrea ; Mlcak, Ron ; Valdivia, Esther ; Yancy, Ryan ; McClure, Matthew L. ; Kotov, Nicholas A. / In vitro analog of human bone marrow from 3D scaffolds with biomimetic inverted colloidal crystal geometry. In: Biomaterials. 2009 ; Vol. 30, No. 6. pp. 1071-1079.
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