In vitro cultivation of Wolbachia pipientis in an Aedes albopictus cell line

Scott L. O'Neill, M. M. Pettigrew, S. P. Sinkins, H. R. Braig, T. G. Andreadis, R. B. Tesh

Research output: Contribution to journalArticle

122 Citations (Scopus)

Abstract

A continuous cell line, Aa23, was established from eggs of a strain of the Asian tiger mosquito, Aedes albopictus, naturally infected with the intracellular aymblont Wolbachia pipientis. The resulting cell line was shown to be persistently infected with the bacterial endosymbiont. Treatment with antibiotics cured the cells of the infection. In the course of establishing this cell line it was noticed that RFLPs in the PCR products of two Wolbachia genes from the parental mosquitoes were fixed in the infected cell line. This indicates that the mosquito host was naturally superinfected with different Wolbachia strains, whereas the infected cell line derived from these mosquitoes only contained one of the original Wolbachia strains. The development of an in vitro culture system for this fastidious microorganism should facilitate molecular analysis of the reproduction distorting phenotypes it induces in natural arthropod hosts.

Original languageEnglish (US)
Pages (from-to)33-39
Number of pages7
JournalInsect Molecular Biology
Volume6
Issue number1
StatePublished - Feb 1997

Fingerprint

Wolbachia pipientis
Wolbachia
Aedes albopictus
Aedes
Culicidae
Cells
cell lines
Cell Line
Arthropods
endosymbionts
in vitro culture
Restriction Fragment Length Polymorphisms
Microorganisms
Eggs
Reproduction
arthropods
restriction fragment length polymorphism
Genes
antibiotics
In Vitro Techniques

Keywords

  • Aedes albopictus
  • cell line
  • in vitro culture
  • Wolbachia pipientis

ASJC Scopus subject areas

  • Insect Science
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry

Cite this

O'Neill, S. L., Pettigrew, M. M., Sinkins, S. P., Braig, H. R., Andreadis, T. G., & Tesh, R. B. (1997). In vitro cultivation of Wolbachia pipientis in an Aedes albopictus cell line. Insect Molecular Biology, 6(1), 33-39.

In vitro cultivation of Wolbachia pipientis in an Aedes albopictus cell line. / O'Neill, Scott L.; Pettigrew, M. M.; Sinkins, S. P.; Braig, H. R.; Andreadis, T. G.; Tesh, R. B.

In: Insect Molecular Biology, Vol. 6, No. 1, 02.1997, p. 33-39.

Research output: Contribution to journalArticle

O'Neill, SL, Pettigrew, MM, Sinkins, SP, Braig, HR, Andreadis, TG & Tesh, RB 1997, 'In vitro cultivation of Wolbachia pipientis in an Aedes albopictus cell line', Insect Molecular Biology, vol. 6, no. 1, pp. 33-39.
O'Neill SL, Pettigrew MM, Sinkins SP, Braig HR, Andreadis TG, Tesh RB. In vitro cultivation of Wolbachia pipientis in an Aedes albopictus cell line. Insect Molecular Biology. 1997 Feb;6(1):33-39.
O'Neill, Scott L. ; Pettigrew, M. M. ; Sinkins, S. P. ; Braig, H. R. ; Andreadis, T. G. ; Tesh, R. B. / In vitro cultivation of Wolbachia pipientis in an Aedes albopictus cell line. In: Insect Molecular Biology. 1997 ; Vol. 6, No. 1. pp. 33-39.
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