In vitro detection of cytotoxicity using FluoroJade-C

Qiang Gu; Susan Lantz-McPeak; Hector Rosas-Hernandez; Elvis Cuevas; Syed F. Ali; Merle G. Paule; Sumit Sarkar

doi:10.1016/j.tiv.2014.01.007

In vitro detection of cytotoxicity using FluoroJade-C

Qiang Gu, Susan Lantz-McPeak, Hector Rosas-Hernandez, Elvis Cuevas, Syed F. Ali, Merle G. Paule, Sumit Sarkar

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

We describe here a novel method for the determination of cytotoxicity in cell cultures using Fluoro-Jade C (FJ-C). FJ-C has been previously used for the assessment of neurodegeneration in fixed brain tissue samples, and has never been utilized in live cell cultures or in different types of cells other than neurons. In the present study we examined the utility of FJ-C for the determination of cytotoxicity in vitro. Various cell cultures were evaluated including neural stem cells, brain microvessel endothelial cells, and SH-SY5Y, PC12 and MDCK cells. Cytotoxicities induced by toxicants in cell cultures, as determined by the FJ-C labeling, were further confirmed by commonly used cytotoxicity assays. This in vitro approach is simple, fast, and sensitive and, thus, has the potential to augment if not replace currently used cell-based cytotoxicity assays.

Original language	English (US)
Pages (from-to)	469-472
Number of pages	4
Journal	Toxicology in Vitro
Volume	28
Issue number	4
DOIs	https://doi.org/10.1016/j.tiv.2014.01.007
State	Published - Jun 2014
Externally published	Yes

Keywords

Cell culture
Fluorescence microscopy
Toxicity

ASJC Scopus subject areas

Toxicology

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10.1016/j.tiv.2014.01.007

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title = "In vitro detection of cytotoxicity using FluoroJade-C",

abstract = "We describe here a novel method for the determination of cytotoxicity in cell cultures using Fluoro-Jade C (FJ-C). FJ-C has been previously used for the assessment of neurodegeneration in fixed brain tissue samples, and has never been utilized in live cell cultures or in different types of cells other than neurons. In the present study we examined the utility of FJ-C for the determination of cytotoxicity in vitro. Various cell cultures were evaluated including neural stem cells, brain microvessel endothelial cells, and SH-SY5Y, PC12 and MDCK cells. Cytotoxicities induced by toxicants in cell cultures, as determined by the FJ-C labeling, were further confirmed by commonly used cytotoxicity assays. This in vitro approach is simple, fast, and sensitive and, thus, has the potential to augment if not replace currently used cell-based cytotoxicity assays.",

keywords = "Cell culture, Fluorescence microscopy, Toxicity",

author = "Qiang Gu and Susan Lantz-McPeak and Hector Rosas-Hernandez and Elvis Cuevas and Ali, {Syed F.} and Paule, {Merle G.} and Sumit Sarkar",

note = "Funding Information: This work was supported by the U.S. Food and Drug Administration, National Center for Toxicological Research, protocol numbers E746001 and E752401. The views expressed here are those of the authors and not necessarily those of the U.S. Food and Drug Administration. ",

year = "2014",

month = jun,

doi = "10.1016/j.tiv.2014.01.007",

language = "English (US)",

volume = "28",

pages = "469--472",

journal = "Toxicology in Vitro",

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AU - Gu, Qiang

AU - Lantz-McPeak, Susan

AU - Rosas-Hernandez, Hector

AU - Cuevas, Elvis

AU - Ali, Syed F.

AU - Paule, Merle G.

AU - Sarkar, Sumit

N1 - Funding Information: This work was supported by the U.S. Food and Drug Administration, National Center for Toxicological Research, protocol numbers E746001 and E752401. The views expressed here are those of the authors and not necessarily those of the U.S. Food and Drug Administration.

PY - 2014/6

Y1 - 2014/6

N2 - We describe here a novel method for the determination of cytotoxicity in cell cultures using Fluoro-Jade C (FJ-C). FJ-C has been previously used for the assessment of neurodegeneration in fixed brain tissue samples, and has never been utilized in live cell cultures or in different types of cells other than neurons. In the present study we examined the utility of FJ-C for the determination of cytotoxicity in vitro. Various cell cultures were evaluated including neural stem cells, brain microvessel endothelial cells, and SH-SY5Y, PC12 and MDCK cells. Cytotoxicities induced by toxicants in cell cultures, as determined by the FJ-C labeling, were further confirmed by commonly used cytotoxicity assays. This in vitro approach is simple, fast, and sensitive and, thus, has the potential to augment if not replace currently used cell-based cytotoxicity assays.

AB - We describe here a novel method for the determination of cytotoxicity in cell cultures using Fluoro-Jade C (FJ-C). FJ-C has been previously used for the assessment of neurodegeneration in fixed brain tissue samples, and has never been utilized in live cell cultures or in different types of cells other than neurons. In the present study we examined the utility of FJ-C for the determination of cytotoxicity in vitro. Various cell cultures were evaluated including neural stem cells, brain microvessel endothelial cells, and SH-SY5Y, PC12 and MDCK cells. Cytotoxicities induced by toxicants in cell cultures, as determined by the FJ-C labeling, were further confirmed by commonly used cytotoxicity assays. This in vitro approach is simple, fast, and sensitive and, thus, has the potential to augment if not replace currently used cell-based cytotoxicity assays.

KW - Cell culture

KW - Fluorescence microscopy

KW - Toxicity

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AN - SCOPUS:84893706067

SN - 0887-2333

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EP - 472

JO - Toxicology in Vitro

JF - Toxicology in Vitro

IS - 4

ER -

In vitro detection of cytotoxicity using FluoroJade-C

Abstract

Keywords

ASJC Scopus subject areas

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