In vitro evidence for pancreatic lineage: Ngn3 positive cells are endocrine progenitors derived from cultured islets

Lu jun Song, Xin yu Qin, Wei xin Niu, Kun tang Shen, Feng lin Liu, K. A. Andreoni, D. A. Gerber, Jeffrey Fair, L. Rice, A. Pleasant, J. Wang

Research output: Contribution to journalArticle

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Abstract

OBJECTIVE: Further studies have been conducted to evaluate the roles of Ngn3 in adult islet maintenance and renewal. METHODS: Islets were isolated from 6 - 8 week old male C57BL/6 mice. After common bile duct cannulation, the pancreas was resected and digested in collagenase V (2.5 mg/ml). Islets were then handpicked and 10 - 12 islets were plated in 60 mm culture dish and cultivated with RPMI-1640, which contained 12.5 mmol/L HEPES, 5.2 mmol/L glucose and 2% fetal bovine serum (FBS). Islet cells were analyzed by immunocytochemistry methods for A6, insulin, glucagon, nestin, Ngn3 and 5-bromo-2'-deoxy-uridine (BrdU). RESULTS: The results of these studies indicated that less than 15 percent of proliferated islet cells were Ngn3 expressing cells, in which about one third of the Ngn3 positive cells co-expressed A6. The existence of Ngn3 in cultured islet cells is consistent with the results from other's findings both in embryogenesis and adult islet studies. A significant finding of our study is that the existence of A6 and Ngn3 co-expressing cells in the cultured islet. A6 is a marker for identifying bile duct epithelial cell oriented hepatic progenitor cells. Islet-derived A6 cells are possibly born in the adult pancreatic duct and migrate into islets. A6 cells co-express Ngn3 when these cells commit to endocrine lineage within the islets. More interestingly, islet-derived A6 positive cells have the potential to transdifferentiate into hepatic cells. CONCLUSION: The presence of Ngn3(+) and A6(+) cells in the cultured islets suggests that the four established islet cell types arise from a common endocrine lineage residing within the adult islets. A6 and Ngn3 are useful markers for understanding intra-islet adult stem cell lineages in our future studies. This approach may allow for significant advances in understanding the IPC proliferation and differentiation, and open the possibility of using intra-islet adult stem cells for diabetes treatment.

Original languageEnglish (US)
Pages (from-to)42-45
Number of pages4
JournalZhonghua wai ke za zhi [Chinese journal of surgery]
Volume43
Issue number1
StatePublished - 2005
Externally publishedYes

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Endocrine Cells
Stem Cells
Islets of Langerhans
Cultured Cells
Adult Stem Cells
Hepatocytes
HEPES
Nestin
Pancreatic Ducts
Common Bile Duct
Collagenases
Cell Lineage
Bromodeoxyuridine
Bile Ducts
Glucagon
Inbred C57BL Mouse
Catheterization
Embryonic Development
In Vitro Techniques
Pancreas

Cite this

Song, L. J., Qin, X. Y., Niu, W. X., Shen, K. T., Liu, F. L., Andreoni, K. A., ... Wang, J. (2005). In vitro evidence for pancreatic lineage: Ngn3 positive cells are endocrine progenitors derived from cultured islets. Zhonghua wai ke za zhi [Chinese journal of surgery], 43(1), 42-45.

In vitro evidence for pancreatic lineage : Ngn3 positive cells are endocrine progenitors derived from cultured islets. / Song, Lu jun; Qin, Xin yu; Niu, Wei xin; Shen, Kun tang; Liu, Feng lin; Andreoni, K. A.; Gerber, D. A.; Fair, Jeffrey; Rice, L.; Pleasant, A.; Wang, J.

In: Zhonghua wai ke za zhi [Chinese journal of surgery], Vol. 43, No. 1, 2005, p. 42-45.

Research output: Contribution to journalArticle

Song, LJ, Qin, XY, Niu, WX, Shen, KT, Liu, FL, Andreoni, KA, Gerber, DA, Fair, J, Rice, L, Pleasant, A & Wang, J 2005, 'In vitro evidence for pancreatic lineage: Ngn3 positive cells are endocrine progenitors derived from cultured islets', Zhonghua wai ke za zhi [Chinese journal of surgery], vol. 43, no. 1, pp. 42-45.
Song, Lu jun ; Qin, Xin yu ; Niu, Wei xin ; Shen, Kun tang ; Liu, Feng lin ; Andreoni, K. A. ; Gerber, D. A. ; Fair, Jeffrey ; Rice, L. ; Pleasant, A. ; Wang, J. / In vitro evidence for pancreatic lineage : Ngn3 positive cells are endocrine progenitors derived from cultured islets. In: Zhonghua wai ke za zhi [Chinese journal of surgery]. 2005 ; Vol. 43, No. 1. pp. 42-45.
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abstract = "OBJECTIVE: Further studies have been conducted to evaluate the roles of Ngn3 in adult islet maintenance and renewal. METHODS: Islets were isolated from 6 - 8 week old male C57BL/6 mice. After common bile duct cannulation, the pancreas was resected and digested in collagenase V (2.5 mg/ml). Islets were then handpicked and 10 - 12 islets were plated in 60 mm culture dish and cultivated with RPMI-1640, which contained 12.5 mmol/L HEPES, 5.2 mmol/L glucose and 2{\%} fetal bovine serum (FBS). Islet cells were analyzed by immunocytochemistry methods for A6, insulin, glucagon, nestin, Ngn3 and 5-bromo-2'-deoxy-uridine (BrdU). RESULTS: The results of these studies indicated that less than 15 percent of proliferated islet cells were Ngn3 expressing cells, in which about one third of the Ngn3 positive cells co-expressed A6. The existence of Ngn3 in cultured islet cells is consistent with the results from other's findings both in embryogenesis and adult islet studies. A significant finding of our study is that the existence of A6 and Ngn3 co-expressing cells in the cultured islet. A6 is a marker for identifying bile duct epithelial cell oriented hepatic progenitor cells. Islet-derived A6 cells are possibly born in the adult pancreatic duct and migrate into islets. A6 cells co-express Ngn3 when these cells commit to endocrine lineage within the islets. More interestingly, islet-derived A6 positive cells have the potential to transdifferentiate into hepatic cells. CONCLUSION: The presence of Ngn3(+) and A6(+) cells in the cultured islets suggests that the four established islet cell types arise from a common endocrine lineage residing within the adult islets. A6 and Ngn3 are useful markers for understanding intra-islet adult stem cell lineages in our future studies. This approach may allow for significant advances in understanding the IPC proliferation and differentiation, and open the possibility of using intra-islet adult stem cells for diabetes treatment.",
author = "Song, {Lu jun} and Qin, {Xin yu} and Niu, {Wei xin} and Shen, {Kun tang} and Liu, {Feng lin} and Andreoni, {K. A.} and Gerber, {D. A.} and Jeffrey Fair and L. Rice and A. Pleasant and J. Wang",
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T1 - In vitro evidence for pancreatic lineage

T2 - Ngn3 positive cells are endocrine progenitors derived from cultured islets

AU - Song, Lu jun

AU - Qin, Xin yu

AU - Niu, Wei xin

AU - Shen, Kun tang

AU - Liu, Feng lin

AU - Andreoni, K. A.

AU - Gerber, D. A.

AU - Fair, Jeffrey

AU - Rice, L.

AU - Pleasant, A.

AU - Wang, J.

PY - 2005

Y1 - 2005

N2 - OBJECTIVE: Further studies have been conducted to evaluate the roles of Ngn3 in adult islet maintenance and renewal. METHODS: Islets were isolated from 6 - 8 week old male C57BL/6 mice. After common bile duct cannulation, the pancreas was resected and digested in collagenase V (2.5 mg/ml). Islets were then handpicked and 10 - 12 islets were plated in 60 mm culture dish and cultivated with RPMI-1640, which contained 12.5 mmol/L HEPES, 5.2 mmol/L glucose and 2% fetal bovine serum (FBS). Islet cells were analyzed by immunocytochemistry methods for A6, insulin, glucagon, nestin, Ngn3 and 5-bromo-2'-deoxy-uridine (BrdU). RESULTS: The results of these studies indicated that less than 15 percent of proliferated islet cells were Ngn3 expressing cells, in which about one third of the Ngn3 positive cells co-expressed A6. The existence of Ngn3 in cultured islet cells is consistent with the results from other's findings both in embryogenesis and adult islet studies. A significant finding of our study is that the existence of A6 and Ngn3 co-expressing cells in the cultured islet. A6 is a marker for identifying bile duct epithelial cell oriented hepatic progenitor cells. Islet-derived A6 cells are possibly born in the adult pancreatic duct and migrate into islets. A6 cells co-express Ngn3 when these cells commit to endocrine lineage within the islets. More interestingly, islet-derived A6 positive cells have the potential to transdifferentiate into hepatic cells. CONCLUSION: The presence of Ngn3(+) and A6(+) cells in the cultured islets suggests that the four established islet cell types arise from a common endocrine lineage residing within the adult islets. A6 and Ngn3 are useful markers for understanding intra-islet adult stem cell lineages in our future studies. This approach may allow for significant advances in understanding the IPC proliferation and differentiation, and open the possibility of using intra-islet adult stem cells for diabetes treatment.

AB - OBJECTIVE: Further studies have been conducted to evaluate the roles of Ngn3 in adult islet maintenance and renewal. METHODS: Islets were isolated from 6 - 8 week old male C57BL/6 mice. After common bile duct cannulation, the pancreas was resected and digested in collagenase V (2.5 mg/ml). Islets were then handpicked and 10 - 12 islets were plated in 60 mm culture dish and cultivated with RPMI-1640, which contained 12.5 mmol/L HEPES, 5.2 mmol/L glucose and 2% fetal bovine serum (FBS). Islet cells were analyzed by immunocytochemistry methods for A6, insulin, glucagon, nestin, Ngn3 and 5-bromo-2'-deoxy-uridine (BrdU). RESULTS: The results of these studies indicated that less than 15 percent of proliferated islet cells were Ngn3 expressing cells, in which about one third of the Ngn3 positive cells co-expressed A6. The existence of Ngn3 in cultured islet cells is consistent with the results from other's findings both in embryogenesis and adult islet studies. A significant finding of our study is that the existence of A6 and Ngn3 co-expressing cells in the cultured islet. A6 is a marker for identifying bile duct epithelial cell oriented hepatic progenitor cells. Islet-derived A6 cells are possibly born in the adult pancreatic duct and migrate into islets. A6 cells co-express Ngn3 when these cells commit to endocrine lineage within the islets. More interestingly, islet-derived A6 positive cells have the potential to transdifferentiate into hepatic cells. CONCLUSION: The presence of Ngn3(+) and A6(+) cells in the cultured islets suggests that the four established islet cell types arise from a common endocrine lineage residing within the adult islets. A6 and Ngn3 are useful markers for understanding intra-islet adult stem cell lineages in our future studies. This approach may allow for significant advances in understanding the IPC proliferation and differentiation, and open the possibility of using intra-islet adult stem cells for diabetes treatment.

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