In vitro evidence of photoreception in the chick pineal gland and its interaction with the circadian clock controlling N-acetyltransferase (NAT)

C. A. Kasal, J. R. Perez-Polo

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    Abstract

    The response of the circadian rhythm in N-acetyltransferase (NAT) activity to phase-shifted light cycles was examined in vitro in explant cultures of chick (Gallus domesticus) pineal glands. Bisected portions of glands, obtained from birds housed in a light-dark cycle (LD 12:12), were explanted into culture and maintained under one of three light cycles (LD 12:12), the phase of which was either 1) similar to that of the birds' previous cycle, 2) seven hours phase-delayed, or 3) six hours phase-advanced. Following two to three days of exposure to the respective light cycles, cultures were placed into continuous darkness (DD). Sampling from cultures during exposure to DD revealed a circadian rhythm of NAT activity. In each case, the phase of the subsequent rhythm of enzyme activity in DD reflected that of the preceding in vitro light cycle. A distinct phase difference of approximately 180° was observed between cultures exposed to opposite lighting regimes. These results indicate entrainment of the circadian rhythm of NAT activity to the prevailing in vitro light cycle and suggest a direct interaction between pineal photoreception and the circadian 'clock' controlling NAT.

    Original languageEnglish (US)
    Pages (from-to)579-585
    Number of pages7
    JournalJournal of Neuroscience Research
    Volume5
    Issue number6
    StatePublished - 1980

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    Circadian Clocks
    Acetyltransferases
    Pineal Gland
    Photoperiod
    Circadian Rhythm
    Birds
    Darkness
    Lighting
    In Vitro Techniques
    Chickens
    Enzymes

    ASJC Scopus subject areas

    • Neuroscience(all)

    Cite this

    In vitro evidence of photoreception in the chick pineal gland and its interaction with the circadian clock controlling N-acetyltransferase (NAT). / Kasal, C. A.; Perez-Polo, J. R.

    In: Journal of Neuroscience Research, Vol. 5, No. 6, 1980, p. 579-585.

    Research output: Contribution to journalArticle

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    N2 - The response of the circadian rhythm in N-acetyltransferase (NAT) activity to phase-shifted light cycles was examined in vitro in explant cultures of chick (Gallus domesticus) pineal glands. Bisected portions of glands, obtained from birds housed in a light-dark cycle (LD 12:12), were explanted into culture and maintained under one of three light cycles (LD 12:12), the phase of which was either 1) similar to that of the birds' previous cycle, 2) seven hours phase-delayed, or 3) six hours phase-advanced. Following two to three days of exposure to the respective light cycles, cultures were placed into continuous darkness (DD). Sampling from cultures during exposure to DD revealed a circadian rhythm of NAT activity. In each case, the phase of the subsequent rhythm of enzyme activity in DD reflected that of the preceding in vitro light cycle. A distinct phase difference of approximately 180° was observed between cultures exposed to opposite lighting regimes. These results indicate entrainment of the circadian rhythm of NAT activity to the prevailing in vitro light cycle and suggest a direct interaction between pineal photoreception and the circadian 'clock' controlling NAT.

    AB - The response of the circadian rhythm in N-acetyltransferase (NAT) activity to phase-shifted light cycles was examined in vitro in explant cultures of chick (Gallus domesticus) pineal glands. Bisected portions of glands, obtained from birds housed in a light-dark cycle (LD 12:12), were explanted into culture and maintained under one of three light cycles (LD 12:12), the phase of which was either 1) similar to that of the birds' previous cycle, 2) seven hours phase-delayed, or 3) six hours phase-advanced. Following two to three days of exposure to the respective light cycles, cultures were placed into continuous darkness (DD). Sampling from cultures during exposure to DD revealed a circadian rhythm of NAT activity. In each case, the phase of the subsequent rhythm of enzyme activity in DD reflected that of the preceding in vitro light cycle. A distinct phase difference of approximately 180° was observed between cultures exposed to opposite lighting regimes. These results indicate entrainment of the circadian rhythm of NAT activity to the prevailing in vitro light cycle and suggest a direct interaction between pineal photoreception and the circadian 'clock' controlling NAT.

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