In vitro glial responses to nerve growth factor

L. A. Hutton, J. R. Perez-Polo

    Research output: Contribution to journalArticle

    32 Citations (Scopus)

    Abstract

    Nerve growth factor (NGF) stimulates expression of the low affinity neurotrophin receptor p75(NGFR) mRNA in primary cultures of neonatal rat cortical type I astrocytes. Nerve growth factor treatment altered glial morphology in glial fibrillary acidic protein positive (GFAP+) cell cultures derived from newborn (P0) and 3-day-old (P3) rat pups. When P0- or P3- derived primary glial cultures were serum-deprived, in the presence of 200 pM NGF for 5 days, the fiat polygonal glia present in culture assumed a fibrous morphology, an effect not seen in the untreated serum-deprived controls. The NGF effect on astrocytic morphology was blocked by continuous serum treatment. Nerve growth factor did not stimulate astrocytic proliferation under these culture conditions, as assayed by cell cycle analysis using 3H thymidine autoradiography. P0-derived primary glial cultures expressed the signal transducing neurotrophin receptors p145(trkB) and p140(trkA) as determined by reverse transcription-polymerase chain reaction (RT-PCR). RT- PCR products were identified by sequencing or restriction enzyme analysis. Astrocytes internalized 125I-NGF at 37°C but not at 4°C, consistent with energy requirements for internalization. Also, internalization of 125I- NGF was abolished by the addition of a 300-1,000-fold excess of unlabeled NGF. Thus, astroglial cells in culture internalize NGF through a specific receptor-mediated process, express trkA and full-length trkB mRNAs at low levels, and respond to exogenous NGF by expressing a fibrous morphology under serum-free culture conditions.

    Original languageEnglish (US)
    Pages (from-to)185-196
    Number of pages12
    JournalJournal of Neuroscience Research
    Volume41
    Issue number2
    DOIs
    StatePublished - 1995

    Fingerprint

    Nerve Growth Factor
    Neuroglia
    Serum
    Astrocytes
    Cell Culture Techniques
    trkA Receptor
    In Vitro Techniques
    Nerve Growth Factor Receptors
    Nerve Growth Factor Receptor
    Polymerase Chain Reaction
    Restriction Mapping
    Messenger RNA
    Glial Fibrillary Acidic Protein
    Autoradiography
    Thymidine
    Reverse Transcription
    Cell Cycle

    Keywords

    • I-nerve growth factor internalization
    • astrocytes
    • morphology
    • nerve growth factor
    • nerve growth factor receptor

    ASJC Scopus subject areas

    • Neuroscience(all)

    Cite this

    In vitro glial responses to nerve growth factor. / Hutton, L. A.; Perez-Polo, J. R.

    In: Journal of Neuroscience Research, Vol. 41, No. 2, 1995, p. 185-196.

    Research output: Contribution to journalArticle

    Hutton, L. A. ; Perez-Polo, J. R. / In vitro glial responses to nerve growth factor. In: Journal of Neuroscience Research. 1995 ; Vol. 41, No. 2. pp. 185-196.
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