In vitro human bone marrow analog

Clinical potential

Joan Nichols, Jean Niles, Shannon Walls, Joaquin Cortiella

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Bone marrow is the primary site of hematopoiesis in adult humans. Bone marrow can be cultured in vitro but few simple culture systems fully support hematopoiesis beyond a few months. Human bone marrow analogs are long-term in vitro cultures of marrow stromal and hematopoietic stem cells that can be used to produce cells and products normally harvested from human donors. Bone marrow analog systems should exhibit confluence of the stromal cell populations, persistence of hematopoietic progenitor cells, presence of active regions of hematopoiesis and capacity to produce mature cell types for extended periods of time. Although we are still years away from realizing clinical application of products formed by artificial bone marrow analogs, the process of transitioning this research tool from bench to bedside should be fairly straightforward. The most obvious application of artificial marrow would be for production of autologous hematopoietic CD34+ stem cells as a stem cell therapy for individuals experiencing bone marrow failure due to disease or injury. Another logical application is for 'blood farming, a process for large-scale in vitro production of red blood cells, white blood cells or platelets, for transfusion or treatment. Other possibilities include production of nonhematopoietic stem cells such as osteogenic stromal cells, osteoblasts and rare pluripotent stem cells. Bone marrow analogs also have great potential as ex vivo human test systems and could play a critical role in drug discovery, drug development and toxicity testing in the future.

Original languageEnglish (US)
Pages (from-to)289-298
Number of pages10
JournalRegenerative Medicine
Volume5
Issue number2
DOIs
StatePublished - Mar 2010

Fingerprint

Bone
Bone Marrow
Stem cells
Hematopoiesis
Blood
Hematopoietic Stem Cells
Cells
Stromal Cells
Stem Cells
Osteoblasts
Platelets
In Vitro Techniques
Cell culture
Platelet Transfusion
Pluripotent Stem Cells
Toxicity
Drug Discovery
Cell- and Tissue-Based Therapy
Drug-Related Side Effects and Adverse Reactions
Agriculture

Keywords

  • Artificial bone marrow
  • Bioreactor cell culture system
  • Bone marrow analog
  • Bone marrow stromal cell n hematopoietic stem cell n human ex vivo organ models n MAPC n mesenchymal stem cell n multipotent adult progenitor cell

ASJC Scopus subject areas

  • Biomedical Engineering
  • Embryology

Cite this

In vitro human bone marrow analog : Clinical potential. / Nichols, Joan; Niles, Jean; Walls, Shannon; Cortiella, Joaquin.

In: Regenerative Medicine, Vol. 5, No. 2, 03.2010, p. 289-298.

Research output: Contribution to journalArticle

Nichols, Joan ; Niles, Jean ; Walls, Shannon ; Cortiella, Joaquin. / In vitro human bone marrow analog : Clinical potential. In: Regenerative Medicine. 2010 ; Vol. 5, No. 2. pp. 289-298.
@article{d13393d6e4394b89b3cc45cd34fd385b,
title = "In vitro human bone marrow analog: Clinical potential",
abstract = "Bone marrow is the primary site of hematopoiesis in adult humans. Bone marrow can be cultured in vitro but few simple culture systems fully support hematopoiesis beyond a few months. Human bone marrow analogs are long-term in vitro cultures of marrow stromal and hematopoietic stem cells that can be used to produce cells and products normally harvested from human donors. Bone marrow analog systems should exhibit confluence of the stromal cell populations, persistence of hematopoietic progenitor cells, presence of active regions of hematopoiesis and capacity to produce mature cell types for extended periods of time. Although we are still years away from realizing clinical application of products formed by artificial bone marrow analogs, the process of transitioning this research tool from bench to bedside should be fairly straightforward. The most obvious application of artificial marrow would be for production of autologous hematopoietic CD34+ stem cells as a stem cell therapy for individuals experiencing bone marrow failure due to disease or injury. Another logical application is for 'blood farming, a process for large-scale in vitro production of red blood cells, white blood cells or platelets, for transfusion or treatment. Other possibilities include production of nonhematopoietic stem cells such as osteogenic stromal cells, osteoblasts and rare pluripotent stem cells. Bone marrow analogs also have great potential as ex vivo human test systems and could play a critical role in drug discovery, drug development and toxicity testing in the future.",
keywords = "Artificial bone marrow, Bioreactor cell culture system, Bone marrow analog, Bone marrow stromal cell n hematopoietic stem cell n human ex vivo organ models n MAPC n mesenchymal stem cell n multipotent adult progenitor cell",
author = "Joan Nichols and Jean Niles and Shannon Walls and Joaquin Cortiella",
year = "2010",
month = "3",
doi = "10.2217/rme.10.7",
language = "English (US)",
volume = "5",
pages = "289--298",
journal = "Regenerative Medicine",
issn = "1746-0751",
publisher = "Future Medicine Ltd.",
number = "2",

}

TY - JOUR

T1 - In vitro human bone marrow analog

T2 - Clinical potential

AU - Nichols, Joan

AU - Niles, Jean

AU - Walls, Shannon

AU - Cortiella, Joaquin

PY - 2010/3

Y1 - 2010/3

N2 - Bone marrow is the primary site of hematopoiesis in adult humans. Bone marrow can be cultured in vitro but few simple culture systems fully support hematopoiesis beyond a few months. Human bone marrow analogs are long-term in vitro cultures of marrow stromal and hematopoietic stem cells that can be used to produce cells and products normally harvested from human donors. Bone marrow analog systems should exhibit confluence of the stromal cell populations, persistence of hematopoietic progenitor cells, presence of active regions of hematopoiesis and capacity to produce mature cell types for extended periods of time. Although we are still years away from realizing clinical application of products formed by artificial bone marrow analogs, the process of transitioning this research tool from bench to bedside should be fairly straightforward. The most obvious application of artificial marrow would be for production of autologous hematopoietic CD34+ stem cells as a stem cell therapy for individuals experiencing bone marrow failure due to disease or injury. Another logical application is for 'blood farming, a process for large-scale in vitro production of red blood cells, white blood cells or platelets, for transfusion or treatment. Other possibilities include production of nonhematopoietic stem cells such as osteogenic stromal cells, osteoblasts and rare pluripotent stem cells. Bone marrow analogs also have great potential as ex vivo human test systems and could play a critical role in drug discovery, drug development and toxicity testing in the future.

AB - Bone marrow is the primary site of hematopoiesis in adult humans. Bone marrow can be cultured in vitro but few simple culture systems fully support hematopoiesis beyond a few months. Human bone marrow analogs are long-term in vitro cultures of marrow stromal and hematopoietic stem cells that can be used to produce cells and products normally harvested from human donors. Bone marrow analog systems should exhibit confluence of the stromal cell populations, persistence of hematopoietic progenitor cells, presence of active regions of hematopoiesis and capacity to produce mature cell types for extended periods of time. Although we are still years away from realizing clinical application of products formed by artificial bone marrow analogs, the process of transitioning this research tool from bench to bedside should be fairly straightforward. The most obvious application of artificial marrow would be for production of autologous hematopoietic CD34+ stem cells as a stem cell therapy for individuals experiencing bone marrow failure due to disease or injury. Another logical application is for 'blood farming, a process for large-scale in vitro production of red blood cells, white blood cells or platelets, for transfusion or treatment. Other possibilities include production of nonhematopoietic stem cells such as osteogenic stromal cells, osteoblasts and rare pluripotent stem cells. Bone marrow analogs also have great potential as ex vivo human test systems and could play a critical role in drug discovery, drug development and toxicity testing in the future.

KW - Artificial bone marrow

KW - Bioreactor cell culture system

KW - Bone marrow analog

KW - Bone marrow stromal cell n hematopoietic stem cell n human ex vivo organ models n MAPC n mesenchymal stem cell n multipotent adult progenitor cell

UR - http://www.scopus.com/inward/record.url?scp=77949405364&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77949405364&partnerID=8YFLogxK

U2 - 10.2217/rme.10.7

DO - 10.2217/rme.10.7

M3 - Article

VL - 5

SP - 289

EP - 298

JO - Regenerative Medicine

JF - Regenerative Medicine

SN - 1746-0751

IS - 2

ER -