In vitro induction of anti-type 2 T cells by glycyrrhizin

N. Nakajima, T. Utsunomiya, Makiko Kobayashi, David Herndon, R. B. Pollard, Fujio Suzuki

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Glycyrrhizin, (GR), an active component of licorice roots, has been previously shown to induce the generation of anti-type 2 T cells (anti-BI2T cells), which are able to counteract the activity of burn-induced CD8+ type 2 T cells (BI2T cells), in thermally injured and normal mice. In the present study anti-B12T cells were generated in vitro in cultures of spleen cells stimulated with GR. Anti-BI2T cells were induced in vitro when splenic mononuclear cells (SMNC) were stimulated in vitro for 24 to 72h with 0.1-10 μg/ml of GR. Anti-BI2T cell activity was detected when suppressor macrophages (Mφ) were depleted from SMNC after stimulation with GR. However, the GR-stimulated generation of anti-BI2T cells required the participation of Mφ or their sonicated fractions. Anti-BI2T cells induced in vitro by GR were identified as Vicia villosa lectin adherent IFNγ producing CD4+ T cells lacking the ability to produce IL-2, IL-4 or IL-10. These results indicate that anti-BI2T cells are generated by GR in vitro. Mφ are not only necessary for the generation of anti-BI2T cells induced by GR but also inhibit their activity.

Original languageEnglish (US)
Pages (from-to)612-617
Number of pages6
JournalBurns
Volume22
Issue number8
DOIs
StatePublished - Dec 1996

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Glycyrrhizic Acid
Burns
T-Lymphocytes
In Vitro Techniques
Thomsen-Friedenreich antibodies
Glycyrrhiza
Interleukin-4
Interleukin-10
Interleukin-2
Spleen
Cell Culture Techniques
Macrophages

ASJC Scopus subject areas

  • Emergency Medicine
  • Surgery

Cite this

Nakajima, N., Utsunomiya, T., Kobayashi, M., Herndon, D., Pollard, R. B., & Suzuki, F. (1996). In vitro induction of anti-type 2 T cells by glycyrrhizin. Burns, 22(8), 612-617. https://doi.org/10.1016/S0305-4179(96)00053-8

In vitro induction of anti-type 2 T cells by glycyrrhizin. / Nakajima, N.; Utsunomiya, T.; Kobayashi, Makiko; Herndon, David; Pollard, R. B.; Suzuki, Fujio.

In: Burns, Vol. 22, No. 8, 12.1996, p. 612-617.

Research output: Contribution to journalArticle

Nakajima, N, Utsunomiya, T, Kobayashi, M, Herndon, D, Pollard, RB & Suzuki, F 1996, 'In vitro induction of anti-type 2 T cells by glycyrrhizin', Burns, vol. 22, no. 8, pp. 612-617. https://doi.org/10.1016/S0305-4179(96)00053-8
Nakajima N, Utsunomiya T, Kobayashi M, Herndon D, Pollard RB, Suzuki F. In vitro induction of anti-type 2 T cells by glycyrrhizin. Burns. 1996 Dec;22(8):612-617. https://doi.org/10.1016/S0305-4179(96)00053-8
Nakajima, N. ; Utsunomiya, T. ; Kobayashi, Makiko ; Herndon, David ; Pollard, R. B. ; Suzuki, Fujio. / In vitro induction of anti-type 2 T cells by glycyrrhizin. In: Burns. 1996 ; Vol. 22, No. 8. pp. 612-617.
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