In vitro maintenance of Leishmania amastigotes directly from lesions

Advantages and limitations

V. H. Hodgkinson, Lynn Soong

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Leishmania amazonensis (MHOM/BR/77/LTB0016) amastigotes were obtained from mouse cutaneous lesions and maintained in vitro for 48 hr at pH 4.6, 33 C. These organisms were reproducing, capable of transformation to promastigotes, and did not display the promastigote-specific antigen, GP46. In contrast, 97% of the organisms maintained for 24 hr at 31 C, pH 7.3, were positive for GP46. Thus, short-term cultivation of this L. amazonensis strain under appropriate conditions can provide a high yield of amastigotes for various in vivo and in vitro studies. However, the possible interference of host immunoglobin on the surface of these amastigotes needs to be considered because fluorescent-labeled anti-mouse immunoglobulin was detected on 16% of lesion-derived amastigotes even after 114 hr of cultivation.

Original languageEnglish (US)
Pages (from-to)953-956
Number of pages4
JournalJournal of Parasitology
Volume83
Issue number5
DOIs
StatePublished - Oct 1997
Externally publishedYes

Fingerprint

amastigotes
Leishmania
lesion
lesions (animal)
Maintenance
Leishmania amazonensis
promastigotes
antigen
Immunoglobulins
Antigens
Skin
organisms
mice
skin lesions
crossover interference
in vitro studies
immunoglobulins
antigens
In Vitro Techniques
organism

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Parasitology
  • Microbiology

Cite this

In vitro maintenance of Leishmania amastigotes directly from lesions : Advantages and limitations. / Hodgkinson, V. H.; Soong, Lynn.

In: Journal of Parasitology, Vol. 83, No. 5, 10.1997, p. 953-956.

Research output: Contribution to journalArticle

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abstract = "Leishmania amazonensis (MHOM/BR/77/LTB0016) amastigotes were obtained from mouse cutaneous lesions and maintained in vitro for 48 hr at pH 4.6, 33 C. These organisms were reproducing, capable of transformation to promastigotes, and did not display the promastigote-specific antigen, GP46. In contrast, 97{\%} of the organisms maintained for 24 hr at 31 C, pH 7.3, were positive for GP46. Thus, short-term cultivation of this L. amazonensis strain under appropriate conditions can provide a high yield of amastigotes for various in vivo and in vitro studies. However, the possible interference of host immunoglobin on the surface of these amastigotes needs to be considered because fluorescent-labeled anti-mouse immunoglobulin was detected on 16{\%} of lesion-derived amastigotes even after 114 hr of cultivation.",
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