TY - JOUR
T1 - In vitro models for studying trophoblast transcellular transport.
AU - Bode, Claudia J.
AU - Jin, Hong
AU - Rytting, Erik
AU - Silverstein, Peter S.
AU - Young, Amber M.
AU - Audus, Kenneth L.
PY - 2006
Y1 - 2006
N2 - In vitro models have proven to be effective in studying the placental transporters that play a role in the exchange of nutrients, waste products, and drugs between the maternal and fetal circulations. Although primary cultures of trophoblast cells can be used to perform uptake, efflux, and metabolism studies, only the rodent HRP-1 and the human BeWo cell lines have been shown to form confluent monolayers when grown on semi-permeable membranes. Protocols for the revival, maintenance, passage, and growth of BeWo cells for transporter expression and transcellular transport studies are provided.
AB - In vitro models have proven to be effective in studying the placental transporters that play a role in the exchange of nutrients, waste products, and drugs between the maternal and fetal circulations. Although primary cultures of trophoblast cells can be used to perform uptake, efflux, and metabolism studies, only the rodent HRP-1 and the human BeWo cell lines have been shown to form confluent monolayers when grown on semi-permeable membranes. Protocols for the revival, maintenance, passage, and growth of BeWo cells for transporter expression and transcellular transport studies are provided.
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M3 - Article
C2 - 16511984
AN - SCOPUS:33645914433
SN - 1543-1894
VL - 122
SP - 225
EP - 239
JO - Methods in molecular medicine
JF - Methods in molecular medicine
ER -