In vitro models for studying trophoblast transcellular transport.

Claudia J. Bode, Hong Jin, Erik Rytting, Peter S. Silverstein, Amber M. Young, Kenneth L. Audus

Research output: Contribution to journalArticle

73 Citations (Scopus)

Abstract

In vitro models have proven to be effective in studying the placental transporters that play a role in the exchange of nutrients, waste products, and drugs between the maternal and fetal circulations. Although primary cultures of trophoblast cells can be used to perform uptake, efflux, and metabolism studies, only the rodent HRP-1 and the human BeWo cell lines have been shown to form confluent monolayers when grown on semi-permeable membranes. Protocols for the revival, maintenance, passage, and growth of BeWo cells for transporter expression and transcellular transport studies are provided.

Original languageEnglish (US)
Pages (from-to)225-239
Number of pages15
JournalMethods in molecular medicine
Volume122
StatePublished - 2006
Externally publishedYes

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Waste Products
Transcytosis
Primary Cell Culture
Trophoblasts
Rodentia
Maintenance
Mothers
Cell Line
Food
Membranes
Growth
Pharmaceutical Preparations
In Vitro Techniques

Cite this

Bode, C. J., Jin, H., Rytting, E., Silverstein, P. S., Young, A. M., & Audus, K. L. (2006). In vitro models for studying trophoblast transcellular transport. Methods in molecular medicine, 122, 225-239.

In vitro models for studying trophoblast transcellular transport. / Bode, Claudia J.; Jin, Hong; Rytting, Erik; Silverstein, Peter S.; Young, Amber M.; Audus, Kenneth L.

In: Methods in molecular medicine, Vol. 122, 2006, p. 225-239.

Research output: Contribution to journalArticle

Bode, CJ, Jin, H, Rytting, E, Silverstein, PS, Young, AM & Audus, KL 2006, 'In vitro models for studying trophoblast transcellular transport.', Methods in molecular medicine, vol. 122, pp. 225-239.
Bode CJ, Jin H, Rytting E, Silverstein PS, Young AM, Audus KL. In vitro models for studying trophoblast transcellular transport. Methods in molecular medicine. 2006;122:225-239.
Bode, Claudia J. ; Jin, Hong ; Rytting, Erik ; Silverstein, Peter S. ; Young, Amber M. ; Audus, Kenneth L. / In vitro models for studying trophoblast transcellular transport. In: Methods in molecular medicine. 2006 ; Vol. 122. pp. 225-239.
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