In vitro protein synthesis directed by separated transcripts of bacteriophage f1 DNA

Teh sheng Chan, Peter Model, Norton D. Zinder

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

RNA was synthesized by purified Escherichia coli RNA polymerase from covalently closed circular replicative form I DNA isolated from E. coli infected with bacteriophage f1. The transcripts were fractionated on the basis of size, and their template activities studied. Both unfractionated and the largest RNA codes for essentially the same products as are made from f1 replicative form I when the DNA is used as a template in a "coupled" system. Other size classes of RNA fail to code for one or more f1-specific products. Hybridization-competition studies suggest that the largest RNA contains sequences common to the smaller RNAs. These data, together with a consideration of the genetic map of f1, support the hypothesis of Sugiura et al. (1969) that filamentous phage DNA contains a number of promoters and one strong terminator. Our data suggest that the terminator is located between the gene VIII and gene III boundaries. Promoter locations are discussed.

Original languageEnglish (US)
Pages (from-to)369-382
Number of pages14
JournalJournal of Molecular Biology
Volume99
Issue number3
DOIs
StatePublished - Dec 15 1975
Externally publishedYes

Fingerprint

Bacteriophages
RNA
DNA
Proteins
Escherichia coli
DNA-Directed RNA Polymerases
Genes
In Vitro Techniques

ASJC Scopus subject areas

  • Molecular Biology
  • Virology

Cite this

In vitro protein synthesis directed by separated transcripts of bacteriophage f1 DNA. / Chan, Teh sheng; Model, Peter; Zinder, Norton D.

In: Journal of Molecular Biology, Vol. 99, No. 3, 15.12.1975, p. 369-382.

Research output: Contribution to journalArticle

Chan, Teh sheng ; Model, Peter ; Zinder, Norton D. / In vitro protein synthesis directed by separated transcripts of bacteriophage f1 DNA. In: Journal of Molecular Biology. 1975 ; Vol. 99, No. 3. pp. 369-382.
@article{ce724663f22e4450afd84a98472a1b45,
title = "In vitro protein synthesis directed by separated transcripts of bacteriophage f1 DNA",
abstract = "RNA was synthesized by purified Escherichia coli RNA polymerase from covalently closed circular replicative form I DNA isolated from E. coli infected with bacteriophage f1. The transcripts were fractionated on the basis of size, and their template activities studied. Both unfractionated and the largest RNA codes for essentially the same products as are made from f1 replicative form I when the DNA is used as a template in a {"}coupled{"} system. Other size classes of RNA fail to code for one or more f1-specific products. Hybridization-competition studies suggest that the largest RNA contains sequences common to the smaller RNAs. These data, together with a consideration of the genetic map of f1, support the hypothesis of Sugiura et al. (1969) that filamentous phage DNA contains a number of promoters and one strong terminator. Our data suggest that the terminator is located between the gene VIII and gene III boundaries. Promoter locations are discussed.",
author = "Chan, {Teh sheng} and Peter Model and Zinder, {Norton D.}",
year = "1975",
month = "12",
day = "15",
doi = "10.1016/S0022-2836(75)80132-X",
language = "English (US)",
volume = "99",
pages = "369--382",
journal = "Journal of Molecular Biology",
issn = "0022-2836",
publisher = "Academic Press Inc.",
number = "3",

}

TY - JOUR

T1 - In vitro protein synthesis directed by separated transcripts of bacteriophage f1 DNA

AU - Chan, Teh sheng

AU - Model, Peter

AU - Zinder, Norton D.

PY - 1975/12/15

Y1 - 1975/12/15

N2 - RNA was synthesized by purified Escherichia coli RNA polymerase from covalently closed circular replicative form I DNA isolated from E. coli infected with bacteriophage f1. The transcripts were fractionated on the basis of size, and their template activities studied. Both unfractionated and the largest RNA codes for essentially the same products as are made from f1 replicative form I when the DNA is used as a template in a "coupled" system. Other size classes of RNA fail to code for one or more f1-specific products. Hybridization-competition studies suggest that the largest RNA contains sequences common to the smaller RNAs. These data, together with a consideration of the genetic map of f1, support the hypothesis of Sugiura et al. (1969) that filamentous phage DNA contains a number of promoters and one strong terminator. Our data suggest that the terminator is located between the gene VIII and gene III boundaries. Promoter locations are discussed.

AB - RNA was synthesized by purified Escherichia coli RNA polymerase from covalently closed circular replicative form I DNA isolated from E. coli infected with bacteriophage f1. The transcripts were fractionated on the basis of size, and their template activities studied. Both unfractionated and the largest RNA codes for essentially the same products as are made from f1 replicative form I when the DNA is used as a template in a "coupled" system. Other size classes of RNA fail to code for one or more f1-specific products. Hybridization-competition studies suggest that the largest RNA contains sequences common to the smaller RNAs. These data, together with a consideration of the genetic map of f1, support the hypothesis of Sugiura et al. (1969) that filamentous phage DNA contains a number of promoters and one strong terminator. Our data suggest that the terminator is located between the gene VIII and gene III boundaries. Promoter locations are discussed.

UR - http://www.scopus.com/inward/record.url?scp=0016594546&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0016594546&partnerID=8YFLogxK

U2 - 10.1016/S0022-2836(75)80132-X

DO - 10.1016/S0022-2836(75)80132-X

M3 - Article

VL - 99

SP - 369

EP - 382

JO - Journal of Molecular Biology

JF - Journal of Molecular Biology

SN - 0022-2836

IS - 3

ER -