In Vitro reconstitution of calf brain microtubules

Effects of macromolecules

James Lee, Neil Tweedy, Serge N. Timasheff

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

A comparative study has been carried out of the ability to reconstitute into microtubules of tubulins prepared by the Weisenberg and the cycle procedures. It was found that further purification of cycle tubulin by phosphocellulose chromatography made its ability to polymerize identical with that of Weisenberg tubulin. By adding to either tubulin the isolated proteins which copurify with tubulin in the cycle procedure, it is possible to reduce their critical concentrations of microtubule formation to a value identical with that of cycle tubulin. It was demonstrated quantitatively that the effect of these nontubulin proteins could be mimicked by a variety of polycationic molecules, the most effective one being poly(L-lysine). A possible mechanism is described by which growing microtubules could be stabilized by subsequent addition of the nontubulin proteins. The conclusion is drawn that, at present, it is not known whether the proteins which copurify with tubulin are specific components of the microtubule system, or simply artefactually coprecipitated impurities.

Original languageEnglish (US)
Pages (from-to)2783-2790
Number of pages8
JournalBiochemistry
Volume17
Issue number14
StatePublished - 1978
Externally publishedYes

Fingerprint

Tubulin
Macromolecules
Microtubules
Brain
Proteins
Chromatography
In Vitro Techniques
Lysine
Purification
Impurities
Molecules

ASJC Scopus subject areas

  • Biochemistry

Cite this

Lee, J., Tweedy, N., & Timasheff, S. N. (1978). In Vitro reconstitution of calf brain microtubules: Effects of macromolecules. Biochemistry, 17(14), 2783-2790.

In Vitro reconstitution of calf brain microtubules : Effects of macromolecules. / Lee, James; Tweedy, Neil; Timasheff, Serge N.

In: Biochemistry, Vol. 17, No. 14, 1978, p. 2783-2790.

Research output: Contribution to journalArticle

Lee, J, Tweedy, N & Timasheff, SN 1978, 'In Vitro reconstitution of calf brain microtubules: Effects of macromolecules', Biochemistry, vol. 17, no. 14, pp. 2783-2790.
Lee, James ; Tweedy, Neil ; Timasheff, Serge N. / In Vitro reconstitution of calf brain microtubules : Effects of macromolecules. In: Biochemistry. 1978 ; Vol. 17, No. 14. pp. 2783-2790.
@article{b3c4f25f7617476e8ee1580abb16f7cb,
title = "In Vitro reconstitution of calf brain microtubules: Effects of macromolecules",
abstract = "A comparative study has been carried out of the ability to reconstitute into microtubules of tubulins prepared by the Weisenberg and the cycle procedures. It was found that further purification of cycle tubulin by phosphocellulose chromatography made its ability to polymerize identical with that of Weisenberg tubulin. By adding to either tubulin the isolated proteins which copurify with tubulin in the cycle procedure, it is possible to reduce their critical concentrations of microtubule formation to a value identical with that of cycle tubulin. It was demonstrated quantitatively that the effect of these nontubulin proteins could be mimicked by a variety of polycationic molecules, the most effective one being poly(L-lysine). A possible mechanism is described by which growing microtubules could be stabilized by subsequent addition of the nontubulin proteins. The conclusion is drawn that, at present, it is not known whether the proteins which copurify with tubulin are specific components of the microtubule system, or simply artefactually coprecipitated impurities.",
author = "James Lee and Neil Tweedy and Timasheff, {Serge N.}",
year = "1978",
language = "English (US)",
volume = "17",
pages = "2783--2790",
journal = "Biochemistry",
issn = "0006-2960",
publisher = "American Chemical Society",
number = "14",

}

TY - JOUR

T1 - In Vitro reconstitution of calf brain microtubules

T2 - Effects of macromolecules

AU - Lee, James

AU - Tweedy, Neil

AU - Timasheff, Serge N.

PY - 1978

Y1 - 1978

N2 - A comparative study has been carried out of the ability to reconstitute into microtubules of tubulins prepared by the Weisenberg and the cycle procedures. It was found that further purification of cycle tubulin by phosphocellulose chromatography made its ability to polymerize identical with that of Weisenberg tubulin. By adding to either tubulin the isolated proteins which copurify with tubulin in the cycle procedure, it is possible to reduce their critical concentrations of microtubule formation to a value identical with that of cycle tubulin. It was demonstrated quantitatively that the effect of these nontubulin proteins could be mimicked by a variety of polycationic molecules, the most effective one being poly(L-lysine). A possible mechanism is described by which growing microtubules could be stabilized by subsequent addition of the nontubulin proteins. The conclusion is drawn that, at present, it is not known whether the proteins which copurify with tubulin are specific components of the microtubule system, or simply artefactually coprecipitated impurities.

AB - A comparative study has been carried out of the ability to reconstitute into microtubules of tubulins prepared by the Weisenberg and the cycle procedures. It was found that further purification of cycle tubulin by phosphocellulose chromatography made its ability to polymerize identical with that of Weisenberg tubulin. By adding to either tubulin the isolated proteins which copurify with tubulin in the cycle procedure, it is possible to reduce their critical concentrations of microtubule formation to a value identical with that of cycle tubulin. It was demonstrated quantitatively that the effect of these nontubulin proteins could be mimicked by a variety of polycationic molecules, the most effective one being poly(L-lysine). A possible mechanism is described by which growing microtubules could be stabilized by subsequent addition of the nontubulin proteins. The conclusion is drawn that, at present, it is not known whether the proteins which copurify with tubulin are specific components of the microtubule system, or simply artefactually coprecipitated impurities.

UR - http://www.scopus.com/inward/record.url?scp=0017841273&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0017841273&partnerID=8YFLogxK

M3 - Article

VL - 17

SP - 2783

EP - 2790

JO - Biochemistry

JF - Biochemistry

SN - 0006-2960

IS - 14

ER -