In vitro toxicity of topical antimicrobial agents to human fibroblasts

Robert L. McCauley, Hugo A. Linares, Virginia Pelligrini, David Herndon, Martin C. Robson, John P. Heggers

Research output: Contribution to journalArticle

81 Citations (Scopus)

Abstract

Topical antimicrobial agents are essential to optimal burn care. However, exposure of WI-38 human diploid fibroblasts (ATCC CCL 75) and fresh donor human dermal fibroblasts to silver sulfadiazine and mafenide acetate results in a significant reduction in cell proliferation, as determined by hemocytometer cell counts and total matrix protein assays, within 48 hr of exposure. Changes in cellular morphology and progressive deterioration of cytoplasmic organelles and the nucleus are seen with phase-contrast microscopy and transmission electron microscopy. These findings may explain the clinical observation of delayed wound healing after the use of topical antimicrobial agents.

Original languageEnglish (US)
Pages (from-to)267-274
Number of pages8
JournalJournal of Surgical Research
Volume46
Issue number3
DOIs
StatePublished - 1989
Externally publishedYes

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Anti-Infective Agents
Mafenide
Fibroblasts
Silver Sulfadiazine
Phase-Contrast Microscopy
Diploidy
Transmission Electron Microscopy
Organelles
Wound Healing
Cell Count
Cell Proliferation
Observation
Skin
Proteins
In Vitro Techniques
silver acetate

ASJC Scopus subject areas

  • Surgery

Cite this

McCauley, R. L., Linares, H. A., Pelligrini, V., Herndon, D., Robson, M. C., & Heggers, J. P. (1989). In vitro toxicity of topical antimicrobial agents to human fibroblasts. Journal of Surgical Research, 46(3), 267-274. https://doi.org/10.1016/0022-4804(89)90069-3

In vitro toxicity of topical antimicrobial agents to human fibroblasts. / McCauley, Robert L.; Linares, Hugo A.; Pelligrini, Virginia; Herndon, David; Robson, Martin C.; Heggers, John P.

In: Journal of Surgical Research, Vol. 46, No. 3, 1989, p. 267-274.

Research output: Contribution to journalArticle

McCauley, RL, Linares, HA, Pelligrini, V, Herndon, D, Robson, MC & Heggers, JP 1989, 'In vitro toxicity of topical antimicrobial agents to human fibroblasts', Journal of Surgical Research, vol. 46, no. 3, pp. 267-274. https://doi.org/10.1016/0022-4804(89)90069-3
McCauley RL, Linares HA, Pelligrini V, Herndon D, Robson MC, Heggers JP. In vitro toxicity of topical antimicrobial agents to human fibroblasts. Journal of Surgical Research. 1989;46(3):267-274. https://doi.org/10.1016/0022-4804(89)90069-3
McCauley, Robert L. ; Linares, Hugo A. ; Pelligrini, Virginia ; Herndon, David ; Robson, Martin C. ; Heggers, John P. / In vitro toxicity of topical antimicrobial agents to human fibroblasts. In: Journal of Surgical Research. 1989 ; Vol. 46, No. 3. pp. 267-274.
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