@inbook{1a2e565f75f64b04ac1eea16727a67c9,
title = "In Vitro Transcription of Plant miRNA for Structural and Processing Analysis",
abstract = "Plant miRNAs are short gene repressors, ranging in length from 20 to 24 nucleotides, that specifically target mRNAs to effectively promote cleavage or impinge translation. Long primary miRNAs (pri-miRNA) from plants fold into double-stranded RNA structures with stem loop structures. These structures are then processed by an RNase type III enzyme and several other proteins. A functional mature miRNA selects its mRNA target through complementary base pairing. Importantly, plant pri-miRNA are variable in size and structure. Here, we describe a protocol to identify plant pri-miRNA processing intermediates by in vitro T7 RNA polymerase transcription and its purification for enzymatic and chemical footprinting as a tool to analyze the peculiarities of plant miRNA processing.",
keywords = "Denaturing gel electrophoresis, Footprinting, miRNA, pri-miRNA, Processing, Secondary structure, T7 RNA polymerase",
author = "Corina Diaz-Quezada and Noe Baruch-Torres and Trasvi{\~n}a-Arenas, \{Carlos H.\} and Brieba, \{Luis G.\}",
note = "Publisher Copyright: {\textcopyright} The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2025.",
year = "2025",
doi = "10.1007/978-1-0716-4398-3\_6",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "107--116",
booktitle = "Methods in Molecular Biology",
}