In vivo and in vitro effects of human growth hormone on rat intestinal ion transport

A. Guarino, R. B. Canani, M. Iafusco, Antonella Casola, R. Russo, A. Rubino

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

It has been reported that: 1) ovine growth hormone stimulates intestinal water, sodium, and chloride absorption and 2) specific growth hormone receptors are present in the rat intestine. Aims of this work were to investigate the effects of acute administration of hGH on water and ion transport in the rat ileum in vivo and in vitro. In vivo, the absorption rates of water, sodium, chloride, and potassium were determined in the rat perfused ileum, during a basal period and after i.v. administration of 6 μg/kg recombinant DNA-derived hGH. In vitro, electrical parameters were measured before and after the hormone addition to the mucosal or the serosal side of rat ileal mucosa mounted in Ussing chambers. In vivo, growth hormone induced a rapid increase in the absorption rates of water, sodium, chloride, and potassium. In vitro, the serosal, but not the mucosal, addition of growth hormone induced a rapid decrease of transepithelial potential difference and of short-circuit current. The effect was time- and dose-dependent, saturable, but not reversible in the short time. The electrical effect was abolished in the absence of chloride, indicating that it was related, at least in part, to inhibition of basal active chloride secretion. Growth hormone also reduced the short-circuit current increase induced by the secretagogues Escherichia coli heat-stable enterotoxin, theophylline, and calcium ionophore A23187. These results indicate that hGH has a rapid absorptive effect that is related, at least in part, to a direct intestinal antisecretory mechanism. It also reduces active intestinal secretion induced by various secratagogues.

Original languageEnglish (US)
Pages (from-to)576-580
Number of pages5
JournalPediatric Research
Volume37
Issue number5
StatePublished - 1995
Externally publishedYes

Fingerprint

Human Growth Hormone
Ion Transport
Growth Hormone
Sodium Chloride
Water
Ileum
Chlorides
Potassium
Intestinal Secretions
Somatotropin Receptors
Recombinant DNA
Calcium Ionophores
Enterotoxins
Calcimycin
Theophylline
Intestines
Sheep
Mucous Membrane
Hot Temperature
Hormones

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health

Cite this

Guarino, A., Canani, R. B., Iafusco, M., Casola, A., Russo, R., & Rubino, A. (1995). In vivo and in vitro effects of human growth hormone on rat intestinal ion transport. Pediatric Research, 37(5), 576-580.

In vivo and in vitro effects of human growth hormone on rat intestinal ion transport. / Guarino, A.; Canani, R. B.; Iafusco, M.; Casola, Antonella; Russo, R.; Rubino, A.

In: Pediatric Research, Vol. 37, No. 5, 1995, p. 576-580.

Research output: Contribution to journalArticle

Guarino, A, Canani, RB, Iafusco, M, Casola, A, Russo, R & Rubino, A 1995, 'In vivo and in vitro effects of human growth hormone on rat intestinal ion transport', Pediatric Research, vol. 37, no. 5, pp. 576-580.
Guarino A, Canani RB, Iafusco M, Casola A, Russo R, Rubino A. In vivo and in vitro effects of human growth hormone on rat intestinal ion transport. Pediatric Research. 1995;37(5):576-580.
Guarino, A. ; Canani, R. B. ; Iafusco, M. ; Casola, Antonella ; Russo, R. ; Rubino, A. / In vivo and in vitro effects of human growth hormone on rat intestinal ion transport. In: Pediatric Research. 1995 ; Vol. 37, No. 5. pp. 576-580.
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