In vivo ANTI‐NGF induces sprouting of sensory axons in dorsal roots

C. E. Hulsebosch, J. R. Perez‐Polo, R. E. Coggeshall

    Research output: Contribution to journalArticlepeer-review

    35 Scopus citations

    Abstract

    Newborn rats were given subcutaneous injections of antibodies to mouse β ‐NGF (ANTI‐NGF) daily for 1 month. The number of neurons in T4‐T6 dorsal root ganglia (DRG) and the numbers of myelinated and unmyelinated axons in the dorsal roots of the same segments were counted in the ANTI‐NGF animals and in normal littermates. The ANTI‐NGF rats had 38% fewer neurons in thoracic ganglia but 17% more myelinated and 40% more unmyelinated fibers than their untreated littermates. Dorsal root ganglion cells also have a larger average size in the ANTI‐NGF animals, which we interpret as a disproportionate loss of small cells. These data are interpreted as showing that some dorsal root ganglion cells, principally small ones, die when endogenous NGF is inactivated, and that the remaining cells emit more processes than normal. Thus, removal of NGF has what appears to be a paradoxical effect, a reduction in dorsal root ganglion cell numbers but an increase in dorsal root axon numbers. The relation of myelin thickness to fiber diameter is also altered, with small fibers being more thinly myelinated in the ANTI‐NGF group. Thus, Schwann cell‐neuronal interactions are also affected by inactivation of NGF.

    Original languageEnglish (US)
    Pages (from-to)445-451
    Number of pages7
    JournalJournal of Comparative Neurology
    Volume259
    Issue number3
    DOIs
    StatePublished - May 15 1987

    Keywords

    • antibodies to nerve growth factor
    • axonal sprouting
    • nerve growth factor
    • unmyelinated axons

    ASJC Scopus subject areas

    • General Neuroscience

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