In vivo detection of superoxide anion production by the brain using a cytochrome c electrode

R. H. Fabian, Douglas Dewitt, T. A. Kent

Research output: Contribution to journalArticle

98 Scopus citations


A cytochrome c-coated platinized carbon electrode was utilized to detect superoxide generated by the brain during hypoxia/hypercarbia, focal ischemia, and reperfusion and following fluid percussion brain injury with and without hemorrhagic hypotension and reperfusion in the rat. All three of these forms of brain injury were associated with an increase in the superoxide signal. The cytochrome c electrode proved to be sensitive and responsive enough for minute-by-minute measurement of superoxide generation by brain tissue.

Original languageEnglish (US)
Pages (from-to)242-247
Number of pages6
JournalJournal of Cerebral Blood Flow and Metabolism
Issue number2
StatePublished - 1995


Cite this