In vivo effects of corticotropin-releasing factor in pregnant rats

V. Jain, S. Q. Shi, Y. P. Vedernikov, George Saade, K. Chwalisz, R. E. Garfield

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

OBJECTIVES: Our purpose was to study the effects of corticotropin- releasing factor on (1) maternal blood pressure (2) uterine vasculature, and (3) parturition in pregnant rats. STUDY DESIGN: infusion minipumps containing vehicle, corticotropin-releasing factor, or α-helical corticotropin- releasing factor 9-41 (corticotropin-releasing factor receptor antagonist) were inserted subcutaneously in timed pregnant rats on day 16 of gestation. Systolic blood pressure was measured daily by the tail-cuff method. The time of onset of labor was determined and the newborn pups were weighed. Circulating levels of corticotropin-releasing factor were measured in untreated controls by radioimmunoassay. Relaxant responses to corticotropin- releasing factor were studied in isolated segments of uterine artery from late (day 18) and term (day 22) pregnant rats mounted in a wire myograph. RESULTS: The blood pressure was decreased by corticotropin-releasing factor and increased by α-helical corticotropin-releasing factor 9-41 (p<0.05). The time of onset of labor was not affected by either treatment. Pup weight was decreased by corticotropin-releasing factor (p < 0.05). Circulating levels of corticotropin-releasing factor (immunoreactive) were not changed in pregnancy. In vitro, corticotropin-releasing factor caused relaxation of the uterine artery in a concentration-dependent manner and the relaxation was decreased at term compared with late pregnancy (p < 0.05). CONCLUSIONS: Endogenously produced corticotropin-releasing factor lowers blood pressure during pregnancy in rats. It is a relaxant of uterine vasculature and this effect is decreased at term. It does not play an essential role in the initiation of labor in rats.

Original languageEnglish (US)
Pages (from-to)186-191
Number of pages6
JournalAmerican Journal of Obstetrics and Gynecology
Volume178
Issue number1 I
DOIs
StatePublished - 1998

Fingerprint

Corticotropin-Releasing Hormone
Blood Pressure
Labor Onset
Uterine Artery
Pregnancy
Corticotropin-Releasing Hormone Receptors
Radioimmunoassay
Tail
Mothers
Parturition
Weights and Measures

Keywords

  • Blood pressure
  • Corticotropin-releasing factor
  • Labor
  • Pregnant rat
  • Uterine artery

ASJC Scopus subject areas

  • Medicine(all)
  • Obstetrics and Gynecology

Cite this

In vivo effects of corticotropin-releasing factor in pregnant rats. / Jain, V.; Shi, S. Q.; Vedernikov, Y. P.; Saade, George; Chwalisz, K.; Garfield, R. E.

In: American Journal of Obstetrics and Gynecology, Vol. 178, No. 1 I, 1998, p. 186-191.

Research output: Contribution to journalArticle

Jain, V. ; Shi, S. Q. ; Vedernikov, Y. P. ; Saade, George ; Chwalisz, K. ; Garfield, R. E. / In vivo effects of corticotropin-releasing factor in pregnant rats. In: American Journal of Obstetrics and Gynecology. 1998 ; Vol. 178, No. 1 I. pp. 186-191.
@article{d05616a110e3491b98b6f23929d9ca30,
title = "In vivo effects of corticotropin-releasing factor in pregnant rats",
abstract = "OBJECTIVES: Our purpose was to study the effects of corticotropin- releasing factor on (1) maternal blood pressure (2) uterine vasculature, and (3) parturition in pregnant rats. STUDY DESIGN: infusion minipumps containing vehicle, corticotropin-releasing factor, or α-helical corticotropin- releasing factor 9-41 (corticotropin-releasing factor receptor antagonist) were inserted subcutaneously in timed pregnant rats on day 16 of gestation. Systolic blood pressure was measured daily by the tail-cuff method. The time of onset of labor was determined and the newborn pups were weighed. Circulating levels of corticotropin-releasing factor were measured in untreated controls by radioimmunoassay. Relaxant responses to corticotropin- releasing factor were studied in isolated segments of uterine artery from late (day 18) and term (day 22) pregnant rats mounted in a wire myograph. RESULTS: The blood pressure was decreased by corticotropin-releasing factor and increased by α-helical corticotropin-releasing factor 9-41 (p<0.05). The time of onset of labor was not affected by either treatment. Pup weight was decreased by corticotropin-releasing factor (p < 0.05). Circulating levels of corticotropin-releasing factor (immunoreactive) were not changed in pregnancy. In vitro, corticotropin-releasing factor caused relaxation of the uterine artery in a concentration-dependent manner and the relaxation was decreased at term compared with late pregnancy (p < 0.05). CONCLUSIONS: Endogenously produced corticotropin-releasing factor lowers blood pressure during pregnancy in rats. It is a relaxant of uterine vasculature and this effect is decreased at term. It does not play an essential role in the initiation of labor in rats.",
keywords = "Blood pressure, Corticotropin-releasing factor, Labor, Pregnant rat, Uterine artery",
author = "V. Jain and Shi, {S. Q.} and Vedernikov, {Y. P.} and George Saade and K. Chwalisz and Garfield, {R. E.}",
year = "1998",
doi = "10.1016/S0002-9378(98)70650-7",
language = "English (US)",
volume = "178",
pages = "186--191",
journal = "American Journal of Obstetrics and Gynecology",
issn = "0002-9378",
publisher = "Mosby Inc.",
number = "1 I",

}

TY - JOUR

T1 - In vivo effects of corticotropin-releasing factor in pregnant rats

AU - Jain, V.

AU - Shi, S. Q.

AU - Vedernikov, Y. P.

AU - Saade, George

AU - Chwalisz, K.

AU - Garfield, R. E.

PY - 1998

Y1 - 1998

N2 - OBJECTIVES: Our purpose was to study the effects of corticotropin- releasing factor on (1) maternal blood pressure (2) uterine vasculature, and (3) parturition in pregnant rats. STUDY DESIGN: infusion minipumps containing vehicle, corticotropin-releasing factor, or α-helical corticotropin- releasing factor 9-41 (corticotropin-releasing factor receptor antagonist) were inserted subcutaneously in timed pregnant rats on day 16 of gestation. Systolic blood pressure was measured daily by the tail-cuff method. The time of onset of labor was determined and the newborn pups were weighed. Circulating levels of corticotropin-releasing factor were measured in untreated controls by radioimmunoassay. Relaxant responses to corticotropin- releasing factor were studied in isolated segments of uterine artery from late (day 18) and term (day 22) pregnant rats mounted in a wire myograph. RESULTS: The blood pressure was decreased by corticotropin-releasing factor and increased by α-helical corticotropin-releasing factor 9-41 (p<0.05). The time of onset of labor was not affected by either treatment. Pup weight was decreased by corticotropin-releasing factor (p < 0.05). Circulating levels of corticotropin-releasing factor (immunoreactive) were not changed in pregnancy. In vitro, corticotropin-releasing factor caused relaxation of the uterine artery in a concentration-dependent manner and the relaxation was decreased at term compared with late pregnancy (p < 0.05). CONCLUSIONS: Endogenously produced corticotropin-releasing factor lowers blood pressure during pregnancy in rats. It is a relaxant of uterine vasculature and this effect is decreased at term. It does not play an essential role in the initiation of labor in rats.

AB - OBJECTIVES: Our purpose was to study the effects of corticotropin- releasing factor on (1) maternal blood pressure (2) uterine vasculature, and (3) parturition in pregnant rats. STUDY DESIGN: infusion minipumps containing vehicle, corticotropin-releasing factor, or α-helical corticotropin- releasing factor 9-41 (corticotropin-releasing factor receptor antagonist) were inserted subcutaneously in timed pregnant rats on day 16 of gestation. Systolic blood pressure was measured daily by the tail-cuff method. The time of onset of labor was determined and the newborn pups were weighed. Circulating levels of corticotropin-releasing factor were measured in untreated controls by radioimmunoassay. Relaxant responses to corticotropin- releasing factor were studied in isolated segments of uterine artery from late (day 18) and term (day 22) pregnant rats mounted in a wire myograph. RESULTS: The blood pressure was decreased by corticotropin-releasing factor and increased by α-helical corticotropin-releasing factor 9-41 (p<0.05). The time of onset of labor was not affected by either treatment. Pup weight was decreased by corticotropin-releasing factor (p < 0.05). Circulating levels of corticotropin-releasing factor (immunoreactive) were not changed in pregnancy. In vitro, corticotropin-releasing factor caused relaxation of the uterine artery in a concentration-dependent manner and the relaxation was decreased at term compared with late pregnancy (p < 0.05). CONCLUSIONS: Endogenously produced corticotropin-releasing factor lowers blood pressure during pregnancy in rats. It is a relaxant of uterine vasculature and this effect is decreased at term. It does not play an essential role in the initiation of labor in rats.

KW - Blood pressure

KW - Corticotropin-releasing factor

KW - Labor

KW - Pregnant rat

KW - Uterine artery

UR - http://www.scopus.com/inward/record.url?scp=0031815026&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031815026&partnerID=8YFLogxK

U2 - 10.1016/S0002-9378(98)70650-7

DO - 10.1016/S0002-9378(98)70650-7

M3 - Article

C2 - 9465827

AN - SCOPUS:0031815026

VL - 178

SP - 186

EP - 191

JO - American Journal of Obstetrics and Gynecology

JF - American Journal of Obstetrics and Gynecology

SN - 0002-9378

IS - 1 I

ER -